Impact of deletion of a catabolite repressor Mig1 on hyphal morphology and cellulase expression in Penicillium funiculosum NCIM1228
Bibliographic record
Abstract
Carbon catabolite repression (CCR) is a regulatory mechanism which negatively regulates genes of for ancillary carbon source utilization. It is mediated by Mig1 orthologues, which are Zn finger transcriptional repressors. We studied the effect of CCR disruption in Penicillium funiculosum NCIM1228, a hypercelluloytic ascomycete. Upon phylogenetic analysis of fungal genomes, Mig1 presence across all taxa of kingdom fungi revealed its conserved role in catabolite repression. Also it was found to constitute distinct clade from industrially important cellulase producing fungi like Trichoderma reesei and Aspergillus sp. It shared the clade with other highly evolved fungi T. cellulolyticus and P. marneffei and represented more recent radiations of evolutionary conserved catabolite repressor Mig1. Genotypic analysis showed that NCIM1228 harbors a truncated yet functional allele of Mig1. Mig1 orthologue of NCIM1228 has a non-sense mutation at 134th amino acid position, making a large C-terminal portion of Mig1 (415aa) dispensable for carbon repression. NCIM1228 was grown in presence of allyl alcohol to check the phenotypic effect. NCIM1228 showed sensitivity to allyl alcohol as compared to Penicillium funiculosum (Pf). Deleting active Zn finger domain made NCIM1228 completely sensitive to allyl alcohol. Surprisingly, the deletion showed small and compact colonies with compromised filamentous proliferation while the dry mycelial weight didn’t change when grown on 0.5% glucose supplemented with 2% avicel. Using microscopy, we unraveled that PfΔMig1 showed reduced aerial hyphae and profuse branching pattern in terminal hyphae resulting in compact colonies. We further observed more than two-fold (7.6 FPU/ml) higher FPU in production media than NCIM1228 under similar condition.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".