Partial purification and sugarcane bagasse induction of extracellular thermostable Amylase by Bacillus sp. under submerged fermentation
Bibliographic record
Abstract
Enzyme with relatively low stability shows an industrial incompetence which creates an urgent need of potential enzymes with high stability in a cost effective way. Thus, thermostable enzymes derived from microorganism are the alternative resources for convenient industrial application. Our present study was aimed for production of thermostable amylase with wide range of pH and temperature stability, and induction of over production of the enzyme by using sugarcane bagasse as the sole carbon source. The strain was an isolate from railway track of Cuttack railway station, Odisha, India. Morphologically the strain was identified as Bacillus species by gram staining, and a potential amylase producer in normal Luria Bertani (LB) broth at 60°C. The optimum extracellular amylase production observed was 0.085 U/ml after 6 hours at 60°C under submerged culture condition. Extracellular amylase was precipitated through ammonium salt fractionation, and partially purified by Sephadex G-50 column chromatography. The partially purified protein under SDS-PAGE was found to be resolved in to three distinct bands of molecular weights approximately 108.69 kDa, 78.12 kDa and 65.63 kDa. The amylase enzyme showed wide range of temperature stability till 90°C and pH stability from pH 5 to 8, respectively. However, the optimum stability of amylase was found at 90°C in pH 8. The induction study has been carried out with abundantly available agro-industrial waste product sugarcane bagasse for over production of amylase in four different concentrations (0.5%, 1.0%, 2.0%, and 5.0%). The enzyme production was enhanced with increase in concentration of sugarcane bagasse as compared to the control LB medium. The highest amylase production observed was 0.190 U/ml with 5.0% inducible substrate after 10 hours of submerged culture condition at 60°C. Thus, the strain is found to be a potential producer of extracellular amylase and further studies are needed for the optimization of increased production and induction of other possible enzymes by different inducible substrates.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".