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Record W2768777089 · doi:10.7717/peerj.4037

The sensitivity of the yeast, <i>Saccharomyces cerevisiae</i>, to acetic acid is influenced by <i>DOM34</i> and <i>RPL36A</i>

2017· article· en· W2768777089 on OpenAlexaff
Bahram Samanfar, Kristina Shostak, Houman Moteshareie, Maryam Hajikarimlou, Sarah Shaikho, Katayoun Omidi, Mohsen Hooshyar, Daniel Burnside, Imelda Galván Márquez, Thomas David Daniel Kazmirchuk, Thet Naing, Paula Ludovico, Anna York-Lyon, Kama E. Szereszewski, Cindy Leung, Jennifer Yixin Jin, Rami Megarbane, Myron L. Smith, Mohan Babu, Martin Holčı́k, Ashkan Golshani

Bibliographic record

VenuePeerJ · 2017
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicFungal and yeast genetics research
Canadian institutionsUniversity of OttawaCarleton UniversityOttawa HospitalUniversity of ReginaChildren's Hospital of Eastern OntarioAgriculture and Agri-Food Canada
Fundersnot available
KeywordsYeastAcetic acidSaccharomyces cerevisiaeHeat shock proteinFermentationBiochemistryHsp70Cell biologyHSP60Heat shockBiologyEthanol fermentationChemistryGene

Abstract

fetched live from OpenAlex

The presence of acetic acid during industrial alcohol fermentation reduces the yield of fermentation by imposing additional stress on the yeast cells. The biology of cellular responses to stress has been a subject of vigorous investigations. Although much has been learned, details of some of these responses remain poorly understood. Members of heat shock chaperone HSP proteins have been linked to acetic acid and heat shock stress responses in yeast. Both acetic acid and heat shock have been identified to trigger different cellular responses including reduction of global protein synthesis and induction of programmed cell death. Yeast HSC82 and HSP82 code for two important heat shock proteins that together account for 1–2% of total cellular proteins. Both proteins have been linked to responses to acetic acid and heat shock. In contrast to the overall rate of protein synthesis which is reduced, the expression of HSC82 and HSP82 is induced in response to acetic acid stress. In the current study we identified two yeast genes DOM34 and RPL36A that are linked to acetic acid and heat shock sensitivity. We investigated the influence of these genes on the expression of HSP proteins. Our observations suggest that Dom34 and RPL36A influence translation in a CAP-independent manner.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.094
Threshold uncertainty score0.396

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.270
Teacher spread0.262 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations24
Published2017
Admission routes1
Has abstractyes

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Same venuePeerJSame topicFungal and yeast genetics researchFrench-language works237,207