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Record W2782654301 · doi:10.1038/s41467-017-01995-2

Identification of genetic elements in metabolism by high-throughput mouse phenotyping

2018· article· en· W2782654301 on OpenAlexafffund
Jan Rozman, Birgit Rathkolb, Manuela A. Oestereicher, Christine Schütt, Aakash Chavan Ravindranath, Stefanie Leuchtenberger, Sapna Sharma, Martin Kistler, Monja Willershäuser, Robert Brommage, Terrence F. Meehan, Jeremy Mason, Hamed Haselimashhadi, Juan Antonio Aguilar‐Pimentel, Lore Becker, Irina Treise, Kristin Moreth, Lillian Garrett, Sabine M. Hölter, Annemarie Zimprich, Susan Marschall, Oana V. Amarie, Julia Calzada-Wack, Frauke Neff, Laura Brachthäuser, Christoph Lengger, Claudia Stoeger, Lilly Zapf, Yi-Li Cho, Patricia da Silva‐Buttkus, Markus Kraiger, Philipp Mayer‐Kuckuk, Karen Kristine Gampe, Moya Wu, Nathalie Conte, Jonathan Warren, Chao-Kung Chen, Ilinca Tudose, Mike Relac, Peter Matthews, Heather Cater, Helen P. M. Natukunda, James Cleak, Lydia Teboul, Sharon Clementson-Mobbs, Zsombor Szoke-Kovacs, Alison P. Walling, Sara Johnson, Gemma Codner, Tanja Fiegel, Natalie Ring, Henrik Westerberg, Simon Greenaway, Duncan Sneddon, Hugh W. Morgan, Jorik Loeffler, Michelle Stewart, Ramiro Ramírez‐Solis, Allan Bradley, William C. Skarnes, Karen P. Steel, Simon A. Maguire, Joshua Dench, David Lafont, Valerie E. Vancollie, Selina Pearson, Amy S. Gates, Mark Sanderson, Carl Shannon, Lauren F. E. Anthony, Maksymilian T. Sumowski, Robbie S. B. McLaren, Brendan Doe, Hannah Wardle‐Jones, Mark Griffiths, Antonella Galli, Agnieszka Świątkowska, Christopher Isherwood, Anneliese O. Speak, Emma L. Cambridge, Heather Wilson, Susana Caetano, Anna Karin B. Maguire, David J. Adams, Joanna Bottomley, Edward J. Ryder, Diane Gleeson, Laurent Pouilly, Stéphane Rousseau, Aurélie Auburtin, Patrick T. Reilly, Abdel Ayadi, Mohammed Selloum, Joshua A. Wood, Dave Clary, Peter J. Havel, Todd Tolentino, Heather Tolentino, Mike Schuchbauer, Sheryl Pedroia, Amanda Trainor, Esi Djan, Milton Pham, Alison Huynh, Vincent de Vera, John Seavitt, Juan Gallegos, Arturo Garza, Elise Mangin, Joel Senderstrom, Iride Lazo, Kate Mowrey, Ritu Bohat, Rodney C. Samaco, Surabi Veeraragavan, Christine Beeton, Sowmya Kalaga, Lois Kelsey, Igor Vukobradovic, Zorana Berberovic, Celeste Owen, Dawei Qu, Ruolin Guo, Susan Newbigging, Lily Morikawa, Napoleon Law, Xueyuan Shang, Patricia Feugas, Yanchun Wang, Mohammad Eskandarian, Yingchun Zhu, Patricia Penton, Valerie Laurin, Shannon Clarke, Qing Lan, Gillian Sleep, Amie Creighton, Elsa Jacob, Ozge Danisment, Marina Gertsenstein, Monica Pereira, S. MacMaster, Sandra Tondat, Tracy Carroll, Jorge Cabezas, Jane Hunter, Greg Clark, Mohammed Bubshait, David Miller, Khondoker Sohel, Hibret A. Adissu, Milan Ganguly, Alexandr Bezginov, Francesco Chiani, Chiara Di Pietro, Gianfranco Di Segni, Olga Ermakova, Filomena Ferrara, Paolo Fruscoloni, Alessia Gambadoro, Serena Gastaldi, Elisabetta Golini, Gina La Sala, Silvia Mandillo, Daniela Marazziti, Marzia Massimi, Rafaele Matteoni, Tiziana Orsini, Miriam Pasquini, Marcello Raspa, Aline Rauch, Gianfranco Rossi, Nicoletta Rossi, Sabrina Putti, Ferdinando Scavizzi, Giuseppe D. Tocchini-Valentini, Shigeharu Wakana, Tomohiro Suzuki, Masaru Tamura, Hideki Kaneda, Tamio Furuse, Kimio Kobayashi, Ikuo Miura, Ikuko Yamada, Yuichi Obata, Atsushi Yoshiki, Shinya Ayabe, J. Nicole Chambers, Karel Chalupský, Claudia Seisenberger, Antje Bürger, Joachim Beig, Ralf Kühn, Andreas Hörlein, Joel Schick, Oskar Oritz, Florian Giesert, Jochen Graw, Markus Ollert, Carsten B. Schmidt‐Weber, Tobias Stoeger, Ali Önder Yildirim, Oliver Eickelberg, Thomas Klopstock, Dirk H. Busch, Raffi Bekeredjian, Andreas Zimmer, Damian Smedley, Mary E. Dickinson, Frank Benso, Iva Morse, Hyoung-Chin Kim, Ho Lee, Soo Young Cho, Tertius Hough, Ann‐Marie Mallon, Sara Wells, Luís Santos, Christopher J. Lelliott, Jacqueline K. White, Tania Sorg, Marie‐France Champy, Lynette Bower, Corey Reynolds, Ann M. Flenniken, Stephen A. Murray, Lauryl M. J. Nutter, Karen L. Svenson, Glauco P. Tocchini‐Valentini, Arthur L. Beaudet, Fátima Bosch, Robert Braun, Michael S. Dobbie, Xiang Gao, Yann Hérault, Ala Moshiri, Bret A. Moore, K. C. Kent Lloyd, Colin McKerlie, Hiroshi Masuya, Nobuhiko Tanaka, Paul Flicek, Helen Parkinson, Radislav Sedláček, Je Kyung Seong, Chi‐Kuang Leo Wang, Mark W. Moore, Steve D. M. Brown, Matthias H. Tschöp, Wolfgang Wurst, Martin Klingenspor, Eckhard Wolf, Johannes Beckers, Fausto Machicao, Andreas Peter, Harald Staiger, Hans‐Ulrich Häring, Harald Grallert, Mónica Campillos, Holger Maier, Helmut Fuchs, Valerie Gailus-Durner, Thomas Werner, Martin Hrabě de Angelis

Bibliographic record

VenueNature Communications · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMetabolomics and Mass Spectrometry Studies
Canadian institutionsLunenfeld-Tanenbaum Research InstituteToronto Centre for PhenogenomicsHospital for Sick Children
FundersFP7 HealthNational Center for Research ResourcesNational Eye InstituteNational Institute of Diabetes and Digestive and Kidney DiseasesBiotechnology and Biological Sciences Research CouncilMedical Research CouncilCentre National de la Recherche ScientifiqueBundesministerium für Bildung und ForschungUniversité de StrasbourgInstitut National de la Santé et de la Recherche MédicaleNational Human Genome Research InstituteWellcome TrustAgence Nationale de la RecherchePHENOMINGovernment of CanadaGenome CanadaNational Institutes of HealthOntario GenomicsNational Research FoundationINFRAFRONTIERAustralian Government
KeywordsIdentification (biology)Computational biologyThroughputGeneticsBiologyComputer science

Abstract

fetched live from OpenAlex

Metabolic diseases are a worldwide problem but the underlying genetic factors and their relevance to metabolic disease remain incompletely understood. Genome-wide research is needed to characterize so-far unannotated mammalian metabolic genes. Here, we generate and analyze metabolic phenotypic data of 2016 knockout mouse strains under the aegis of the International Mouse Phenotyping Consortium (IMPC) and find 974 gene knockouts with strong metabolic phenotypes. 429 of those had no previous link to metabolism and 51 genes remain functionally completely unannotated. We compared human orthologues of these uncharacterized genes in five GWAS consortia and indeed 23 candidate genes are associated with metabolic disease. We further identify common regulatory elements in promoters of candidate genes. As each regulatory element is composed of several transcription factor binding sites, our data reveal an extensive metabolic phenotype-associated network of co-regulated genes. Our systematic mouse phenotype analysis thus paves the way for full functional annotation of the genome.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.233
Threshold uncertainty score0.427

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.288
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations72
Published2018
Admission routes2
Has abstractyes

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