Characterization of a functional insertion sequence ISSau2 from Staphylococcus aureus
Bibliographic record
Abstract
IS Sau2 has been suggested as a member of the IS 150 f subgroup in the IS3 family. It encodes a fusion transposase OrfAB produced by programmed − 1 translational frameshifting with two overlapping reading frames orfA and orfB . To better characterize IS Sau2 , the binding and cleaving activities of the IS Sau2 transposase and its transposition frequency were studied. The purified IS Sau2 transposase OrfAB was a functional protein in vitro since it bound specifically to IS Sau2 terminal inverted repeat sequences (IRs) and cleaved the transposon ends at the artificial mini-transposon pUC19-IRL-gfp-IRR. In addition, the transposition frequency of IS Sau2 in vivo was approximately 1.76 ± 0.13 × 10 − 3 , based on a GFP hop-on assay. Furthermore, OrfB cleaved IRs with the similar catalytic activity of OrfAB, while OrfA had no catalytic activity. Finally, either OrfA or OrfB significantly reduced the transposition of IS Sau2 induced by OrfAB. We have confirmed that IS Sau2 is a member of IS 150 /IS 3 family. The IS Sau2 transposase OrfAB could bind to and cleave the specific fragments containing the terminal inverted repeat sequences and induce the transposition, suggesting that IS Sau2 is at least partially functional. Meanwhile, both OrfA and OrfB inhibited the transposition by IS Sau2 . Our results will help understand biological roles of IS Sau2 in its host S. aureus .
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".