Teucrium polium extracts stimulate GLUT4 translocation to the plasma membrane in L6 muscle cells
Bibliographic record
Abstract
Teucrium polium L. (TP) is recommended by herbal and integrative practitioners for the treatment of diabetes.However, its mechanism of action in stimulating GLUT4 activity and translocation to the plasma membrane is still unknown.This in vitro study examined the chemical composition, cytotoxicity, and antidiabetic activity of three TP distinct extracts: water/ethanol (WTP), methanol (MTP), and hexane (HTP).The compositions of the TP extracts were determined by GC/MS.MTT and LDH assays were used to assess the toxicity of the extracts.The efficacies of the TP extracts in enhancing glucose transporter-4 (GLUT4) translocation to plasma membrane (PM) were tested in L6 muscle cells, stably expressing myctagged GLUT4 using the cell-ELISA test.GC/MS phytochemical analysis of MTP and HTP extracts revealed 10 compounds in each extract, and only palmitic acid was communal in these two extracts.WTP, MTP, and HTP extracts were safe up to 63, 63, and 250 g/ml, respectively.The HTP extract was the most efficient in GLUT4 translocation enhancement, while the least efficient was the WTP extract.In addition, the HTP extract increased the GLUT4 translocation at 32 g/ml by 2-and 3-fold relative to the control in the absence and presence of insulin, respectively.A similar result was obtained with the MTP extract at 63 g/ml.In contrast, WTP extract in the presence of insulin had no effect and in the absence of insulin had slightly enhanced GLUT4 translocation.These findings indicate that TP antidiabetic activity is mediated in part by enhancing GLUT4 translocation to the PM in skeletal muscle.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".