A310 DAILY ADMINISTRATION OF LACTOBACILLUS PLANTARUM IMPROVES MOUSE JUVENILE GROWTH KINETICS BY SUSTAINING SOMATOTROPIC AXIS ACTIVITY UPON UNDERNUTRITION.
Bibliographic record
Abstract
Chronic undernutrition, a condition currently affecting more than 17 millions of children under five years of age, has severe long-term consequences including stunting. Epidemiologic studies have emphasized that undernutrition cannot be ascribed to food insecurity alone and gut microbiota has been shown to play an active role in disease aetiology. In mammals, post-natal growth is controlled by the activity of the somatotropic axis. Undernutrition leads to a decrease of the Insulin like Growth Factor 1 (IGF-1) and a state of Growth Hormon (GH) resistance. Previously we have shown the capacity of selected Lactobacillus plantarum strain to maintain growth in infant mono-colonized mice during chronic undernutrition. Here we show that L. plantarum retains its growth promoting capabilities also in a conventional mouse model. C57BL6 male mice were weaned at 21 days and bred on a standard or an experimental (isocaloric, hypoprotidic and hypolipidic) diet until young adulthood. One group of mice on experimental diet received an LpWJL oral supplementation (2*10^8 CFU/day; 5 days per week) and the other group received placebo. Length and weight were measured weekly. Mice were sacrificed at day 56 to study the impact of the LpWJL oral supplementation on IGF-1 levels and organ growth. Somatotropic axis activity was tested at Day 28 by injecting the mice with GH and measured by the STAT5 phosphorylation level. At D56, mice fed with the experimental diet were smaller than the standard diet group (7.7 vs 8.9 cm; p< 0.01). Undernourished mice had a lower hepatic level of IGF-1 (113 ± 39 vs 174 ± 35 pg/mg tissues; p<0.01) and a lower plasmatic level of IGF-1 (150 ± 50 vs 388 ± 103 ng/mL; p< 0.01) compared with the standard diet group. Body length of mice fed experimental diet was longer in LpWJL –supplemented group compared to the placebo supplementation (8.02 ± 0.19 vs 7.73 ± 0.16 cm; p<0.0001). LpWJL-treated mice showed 23% increase in daily growth gain compared to placebo without the change in the mean daily food intake. In the LpWJL group, mice had a higher hepatic IGF-1 level (108 ± 12.5 vs 59.8 ± 18.5 pg/mg tissues; p< 0.0001) and a higher plasmatic IGF-1 level (209 ± 51 vs 148 ± 32 ng/mL; p< 0.001) compared to the placebo group. At day 28, mice exposed to LpWJL during starvation process showed an increase of the sensibility of the hepatic GH receptor to GH according to the STAT5 phosphorylation level. Oral supplementation by LpWJL alleviates the GH resistance and improves juvenile growth of conventional infant mice upon undernutrition. None
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".