A287 PHYSICAL ACTIVITY AS A MODULATOR OF INTESTINAL MICROBIOTA, IMMUNE RESPONSES, AND SHORT-CHAIN FATTY ACIDS PRODUCTION
Bibliographic record
Abstract
The trillions of bacteria residing along the mammalian intestine, referred to as the gut’s microbiome, are integral in various physiological function of their host. Disruptions in these communities, or dysbiosis, are implicated in various illnesses such as inflammatory bowel disease and colorectal cancer. Many factors such as diet, method of birth, and environmental exposures are known to influence the intestinal microbiota, however the role of physical activity (PA) remains unclear. A) To explore the relationship between cardiorespiratory fitness (CRF) and gut microbiota in humans and; B) confirm causation between PA and the microbiota using a mouse model of PA A) Using high-throughput DNA sequencing, we compared fecal microbiota of 39 individuals with varying CRF level as determined by peak oxygen uptake test (VO2peak). Fecal short-chain fatty acids (SCFA) were analysed using GC. B) Female C57BL/6 mice were randomly categorized into voluntary wheel running (VWR), or sedentary (SED) group (n=8). Intestinal bacterial community, morphology, and gene expression of various cytokines was analyzed after 7 weeks. A) VO2peak in humans was significantly correlated with increased microbial biodiversity and distinct metagenomic functions. The microbial profiles of fit individuals were associated with increased production of buryate, a SCFA known for its anti-inflammatory properties, through increased abundances of key butyrate-producing taxa: Clostridiales, Roseburia, Lachnospiraceae, and Erysipelotrichaceae. B) VWR mice gained weight at a significantly higher rate than SED group, absent changes in their food intake. Firmicutes phyla was significantly higher (29.0%, p<0.05) in VWR mice while Bacteroidetes was higher (260%, p<0.05) among SED mice. VWR had a higher Firmicutes to Bacteroidetes ratio, previously associated with increased energy harvest and weight gain. Histological analysis revealed no group differences in the number of goblet cells, crypt lengths, or total muscularis externa. A significance 2.1 and 1.9 fold increases in gene expression of the cytokines TGFβ and TNFα, respectively, was observed among the SED mice suggesting immunogenic differences induced by PA. VO2peak in humans was correlated with increased microbial diversity and production of butyrate through increases in several key butyrate-producing taxa. Further, wheel running in mice increased the Firmicutes to Bacteroidetes phyla ratio, likely leading to increased energy harvest and weight gain without changes in food intake. Wheel running was also associated with suppressed gene expression of the pro-inflammatory cytokine TNFα, and TGFβ, a marker of cell proliferation, suggesting an overall healthier gut among physically active mice. CCCNSERC
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".