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Record W2791233146 · doi:10.1002/dvdy.24622

P63 expression plays a role in developmental rate, embryo size, and local morphogenesis

2018· article· en· W2791233146 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueDevelopmental Dynamics · 2018
Typearticle
Languageen
FieldMedicine
TopicCancer-related Molecular Pathways
Canadian institutionsUniversity of TorontoHospital for Sick ChildrenUniversity of Saskatchewan
FundersNatural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchHospital for Sick ChildrenCanada Foundation for InnovationUniversity of Saskatchewan
KeywordsMorphogenesisBiologyEmbryoOrganogenesisPhenotypeEmbryogenesisMutantHeterochronyGenitourinary systemCell biologyBrachyuryAnatomyAndrologyGeneticsOntogenyGeneEmbryonic stem cellMesoderm

Abstract

fetched live from OpenAlex

Background: The p63 gene is integral to the development of many body parts including limb, palate, teeth, and urogenital tract. Loss of p63 expression may alter developmental rate, which is crucial to normal morphogenesis. To validate a novel, unbiased embryo phenotyping software tool, we tested whether delayed development contributes to the pathological phenotype of a p63 mouse mutant (p63 ‐/‐ ). We quantified dysmorphology in p63 ‐/‐ embryos and tested for universal growth delay relative to wild‐type (WT) embryos. Fixed embryos (n = 6; p63 ‐/‐ ) aged day (E) 15.5 were micro‐CT scanned and quantitatively analyzed using a digital WT atlas that defined volumetric differences between p63 ‐/‐ and WT embryos. Results: p63 ‐/‐ embryos showed a growth delay of approximately 22 hr (0.9 days). Among the E15.5 mutants, overall size was closest to WT E14.6 mice but shape was closest to WT E14.0. The atlas clearly identified in p63 ‐/‐ embryos malformations of epithelial derivatives including limbs, tail, urogenital structures, brain, face, and tooth. Conclusions: The software atlas technique described the p63 ‐/‐ phenotype as a combination of developmental delay (i.e., heterochrony) and malformation (i.e., pathological shape; failed organogenesis). This study identifies for the first time global and local roles for p63 in prenatal growth and development. Developmental Dynamics 247:779–787, 2018 . © 2018 Wiley Periodicals, Inc.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.565
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.216
Teacher spread0.209 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it