Phylogenetic analysis of microcystin biosynthesis gene-<i>mcyF</i> from <i>Microcystis</i>
Bibliographic record
Abstract
Microcystinは淡水藍藻の一部の種が産生する非リボソ―ムペプチドであり、強力な肝臓毒として知られている。このMicrocystin生合成のためにはD-glutamate と D-MeAsp/D-aspartateをペプチド骨格に組み込むラセマーゼ活性が必要となる。本研究ではmicrocystin合成酵素遺伝子mcyクラスターに位置するラセマーゼ遺伝子mcyFに着目し、microcystin産生藍藻の系統発生について考察を行った。13株のmicrocystin産生藍藻株においてmcyFホモログ遺伝子が確認され、McyFはmicrocystin生合成に関連する酵素と言える。9株のMcyFのアミノ酸解析ならびに系統発生解析を行った。保存されたアミノ酸モチーフの解析結果からMcyFはglutamate racemase よりaspartate racemaseの機能をすることが予想された。系統発生解析の結果から、McyFがaspartate/glutamate racemaseの系統樹において明らかにaspartateのグループに近い位置にあることが判明した。
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".