A309 ANTIBIOTIC DRIVEN CHANGES IN GUT MOTILITY HIGHLIGHT DIRECT MODULATION OF ENTERIC NERVOUS SYSTEM
Bibliographic record
Abstract
The microbiome-gut-brain axis is a bidirectional network that influences brain chemistry and behaviour. Antibiotics are known to alter several aspects of gut-brain signaling but the underlying mechanisms remain equivocal. As prior assessments of these mechanisms have been limited to changes in gut microbiota, we hypothesized that antibiotics may directly modulate neurons and neural reflexes. To identify whether transient exposure of the gastrointestinal tract to antibiotics can acutely modulate peristaltic reflexes that depend on the enteric nervous system (ENS). 4 cm colon segments excised from adult male Swiss Webster mice were submerged in an organ bath chamber (34°C oxygenated Krebs buffer solution) and cannulated at both the oral and anal ends. Gut lumens were perfused with Krebs saline control followed by either Krebs diluted Bacitracin (1, 3 or 10 mM) or Penicillin V (3, 10, 30 or 100 mM). Gut motility was recorded by video which was subsequently converted to spatiotemporal diameter maps for quantitative analysis. Paired t-tests were performed for before and after measurements. Alterations in ENS-dependent propagating contractile cluster (PCC) velocity and frequency were evoked by Bacitracin at concentrations of 1, 3 and 10 mM and Penicillin V above 10 mM. For Bacitracin, PCC velocity (mm/s) increased from 0.23 ± 0.06 to 0.35 ± 0.13 (n=20, P<0.001) at 1 mM, from 0.28 ± 0.11 to 0.33 ± 0.13 (n=20, P= 0.034) at 3 mM and increased from 0.26 ± 0.11 to 0.28 ± 0.11 (n=20, P=0.8) at 10 mM. Similarly, PCC frequency (Hz) increased from 0.009 ± 0.002 to 0.011 ± 0.003 (n=20, P=0.037) at 1 mM, from 0.012 ± 0.003 to 0.013 ± 0.003 (n=20, P=0.05) at 3 mM and remained at 0.01 ± 0.004 Hz (n=20, P=0.9) at 10 mM. Similar results were produced in experiments using >10 mM Penicillin V. PCC velocity (mm/s) increased from 0.21 ± 0.05 to 0.22 ± 0.04 (n=20, P=0.15) at 10 mM, 0.17 ± 0.04 to 0.54 ± 0.2 (n=16, P<0.001) at 30 mM and from 0.17 ± 0.04 to 0.65 ± 0.2 (n=16, P<0.001) at 100 mM. PCC frequency (Hz) increased from 0.01 ± 0.001 to 0.012 ± 0.002 (n=20, P=0.013) at 10 mM, from 0.01 ± 0.002 to 0.04 ± 0.03 (n=16, P=0.008) at 30 mM and from 0.01 ± 0.002 to 0.05 ± 0.03 (n=16, P=0.001) at 100 mM. These experiments suggest that luminal antibiotics acutely alter ENS dependent reflexes. Therefore, studies attributing the effects of antibiotics solely to disruption of the gut microbiota should be interpreted with caution. CIHRInternational Development Research Council (IDRC) Canada-Israel Joint Research Program #26749
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".