A reliable rapid protocol for characterization of in vitro totipotency in Spilanthes oleracea
Bibliographic record
Abstract
Spilanthes oleracea is an important medicinal herb and is also called ‘Toothache Plant’ or ‘Eye Ball Plant’. It is used to prepare herbal formulation to cure many diseases. Its property to heal the dental wounds can be exploited as an alternative to synthetic medicines used presently. An efficient protocol for in vitro shoot multiplication of Spilanthes oleracea has been developed from axillary bud explants. Nodal segments, from young plants, were taken as explants; shoot multiplication was induced on slightly modified Murashige and Skoog’s (MS) medium supplemented with 6- Benzyl amino purine, (BAP, 0.5 ppm) and Naphthalene Acetic Acid, (NAA, 0.1 ppm) and BAP (0.5 ppm) + Indole 3-Acetic Acid, (IAA, 0.3 ppm). Shoot proliferation could be induced using different combinations of BAP, IAA and NAA. Shoots were further multiplied through continued subculture of nodal segments with sprouted shoots. Micro-shoots were rooted in the basal medium supplemented with NAA (1.71 µM) alone and BAP (0.44 µM) + NAA (1.0 µM) concentration.) Survival of in vitro grown plantlets 2 months after transplantation in the pots, containing equal parts of sand and top soil, was found to be 97 per cent.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".