Genetic characterization of macrophages from induced sputum of asthma and COPD patients
Bibliographic record
Abstract
Methods Sputum induction and processing was proceeded as previously described. 8A FITC Pos itive Selection Kit (StemCell, Vancouver, Cana da) was used for sputum macrophage (SM) sep aration.The immunomagnetic separation of cells labeled with CD68 + FITC antibody (Thermo Fish er, Waltham, Massachusetts, United States) was performed according to the manufacturer's in struction.The number of macrophages in isolat ed cells was evaluated on May-Grünwald Giem sa stained smears (Supplementary material, Table S2).The threshold for macrophage dominated material was arbitrarily set as more than 50% of isolated cells.The total RNA was isolated us ing the Tri reagent/chloroform method (Sigma Aldrich, Saint Louis, Missouri, United States).RNA quality was measured with the Agilent 2100 bioanalyzer (Agilent Technologies
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".