Abstract 20158: Consequences of Co-isolation of Brain and Heart on the Function of Isolated Cardiac Mitochondria in a Swine Model of Cardiopulmonary Resuscitation
Bibliographic record
Abstract
Introduction: Functional testing of isolated mitochondria is a powerful in vitro tool for determining mitochondrial health following an ischemic stress. Successful preservation of intact mitochondria requires an efficient harvest technique under cold conditions. As heart and brain mitochondria are vital to the success of cardiopulmonary resuscitation (CPR), we sought to determine the consequence of co-isolation of these organs on cardiac mitochondrial function in a porcine model of cardiac arrest. Methods: After 15 min of ventricular fibrillation, 36 animals were randomized to active compression decompression + impedance threshold device (ACD-ITD) CPR (Control), or ACD-ITD CPR + ischemic postconditioning (IPC) and further randomized to heart-only (Control n=9, IPC n=11) or heart + brain mitochondrial isolation (Control n=8, IPC n=8). IPC consisted of 3 cycles of 20 sec compression / 20 sec pause for the first 2 min of CPR. Cardiac and brain mitochondria were isolated via differential centrifugation 4 min after initiation of CPR. To minimize warm ischemia, brain biopsies were performed while animals were supine with uninterrupted chest compressions. Heart biopsies were subsequently performed via intercostal incision. Respiratory control index (RCI) and calcium retention capacity (CRC) were measured for complex I and II substrates. Results: Due to technique, heart isolation was delayed by 100 s in heart + brain vs. heart-only animals. In animals with heart-only isolation, IPC increased complex I and II RCI and complex I CRC compared to Controls. In animals with heart + brain isolation, cardiac complex I and II RCI and complex I CRC decreased within the IPC group but not within the Control group. There were no differences in brain mitochondrial function between IPC and Controls. Conclusion: Mitochondrial isolation from multiple organs must consider warm ischemia that may worsen mitochondrial performance. Communication between the brain and heart during biopsy appears to be mediated by a mechanism antagonistic to the beneficial effects of IPC, as isolation of heart and brain resulted in deleterious cardiac mitochondrial performance in IPC but not in Controls. Further investigation is warranted to refine multi-organ mitochondrial isolation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".