A glycoform of the secreted purple acid phosphatase <scp>AtPAP26</scp> co‐purifies with a mannose‐binding lectin (<scp>AtGAL1</scp>) upregulated by phosphate‐starved <i>Arabidopsis</i>
Bibliographic record
Abstract
Abstract The purple acid phosphatase AtPAP26 plays a central role in Pi‐scavenging by Pi‐starved (−Pi) Arabidopsis . Mass spectrometry (MS) of AtPAP26‐S1 and AtPAP26‐S2 glycoforms secreted by −Pi suspension cells demonstrated that N‐glycans at Asn 365 and Asn 422 were modified in AtPAP26‐S2 to form high‐mannose glycans. A 55‐kDa protein that co‐purified with AtPAP26‐S2 was identified as a Galanthus nivalis agglutinin‐related and apple domain lectin‐1 (AtGAL1; At1g78850). MS revealed that AtGAL1 was bisphosphorylated at Tyr 38 and Thr 39 and glycosylated at four conserved Asn residues. When AtGAL was incubated in the presence of a thiol‐reducing reagent prior to immunoblotting, its cross‐reactivity with anti‐AtGAL1‐IgG was markedly attenuated (consistent with three predicted disulfide bonds in AtGAL1's apple domain). Secreted AtGAL1 polypeptides were upregulated to a far greater extent than AtGAL1 transcripts during Pi deprivation, indicating posttranscriptional control of AtGAL1 expression. Growth of a −Pi atgal1 mutant was unaffected, possibly due to compensation by AtGAL1's closest paralog, AtGAL2 (At1g78860). Nevertheless, AtGAL1 's induction by numerous stresses combined with the broad distribution of AtGAL1‐like lectins in diverse species implies an important function for AtGAL1 orthologs within the plant kingdom. We hypothesize that binding of AtPAP26‐S2's high‐mannose glycans by AtGAL1 enhances AtPAP26 function to facilitate Pi‐scavenging by −Pi Arabidopsis .
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".