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Response of CAPN2 to Exercise Intensity and Total Energy Expenditure

2015· article· en· W2890271689 on OpenAlexafffund
A. N. Belcastro

Bibliographic record

VenueThe FASEB Journal · 2015
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCalpain Protease Function and Regulation
Canadian institutionsYork University
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsAnimal scienceEnergy expenditureIntensity (physics)ChemistryInternal medicineSkeletal muscleEndocrinologyExercise intensityPhysical activityMedicinePhysical therapyHeart rateBlood pressureBiologyPhysics

Abstract

fetched live from OpenAlex

The purpose was to investigate whether the response of skeletal muscle calpain (SM‐CAPN2) activity to exercise varies by intensity, energy expenditure (EE) and/or muscle type. Rats were assigned to a control group (CG) and either a lower or higher energy expenditure exercise (LEE and HEE) group, which included three intensity groups (15m/min, 25m/min and 35m/min). Soleus (SOL), plantaris (PL) and vastus lateralis (VL) muscles were assessed for CAPN2 activities. Blood CK and SM‐CAPN2 activity increased in a dose‐dependent manner in response to exercise (r=0.66). Exercise increased SM‐CAPN2 activities from control (13.07 ± 3.51 U/G wet wt), to LEE (15.36 ± 3.40 U/G wet wt) and HEE (24.65 ± 4.21 U/G wet wt) (p<0.05). The greater SM‐CAPN2 activities observed when going from LEE (70 kcal/session) to HEE (140 kcal/session) were similar across all muscles (i.e., SOL = 67.7%, PL = 57.9% and VL 62.2%). For both LEE and HEE SM‐CAPN2 activities increased with exercise intensity in an observable dose‐dependent manner. At 15m/min (50% VO 2 max ) PL CAPN2 activities for the LEE and HEE groups were 14.1 ± 4.2 and 22.5 ± 5.5 U/g wet wt, respectively (p<0.05); at 25m/min (70% VO 2 max ) CAPN2 activities were 17.6 ± 4.4 and 33.8 ± 7.2 U/g wet wt, respectively, for LEE and HEE (p<0.05). Comparable effects of intensity were observed with the VL muscles, but not so for SOL; which had mixed responses to exercise intensity with larger inter‐animal variability. The greater response of SM‐CAPN2 activity with increasing EE and at higher exercise intensities may contribute to the increased protein release and/or protein turnover with physical activity. Supported by NSERC to ANB

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.142

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.230
Teacher spread0.218 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2015
Admission routes2
Has abstractyes

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