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Record W2890785725 · doi:10.3389/fphys.2018.01406

Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations

2018· article· en· W2890785725 on OpenAlexafffund
Julia Fux, Amrit Mehta, Jack Moffat, J. David Spafford

Bibliographic record

VenueFrontiers in Physiology · 2018
Typearticle
Languageen
FieldEarth and Planetary Sciences
TopicMarine Invertebrate Physiology and Ecology
Canadian institutionsUniversity of Waterloo
FundersNatural Sciences and Engineering Research Council of CanadaHeart and Stroke Foundation of Canada
KeywordsGatingSodium channelIon channelBiologyTransmembrane domainBiophysicsCell biologyBiochemistryChemistryGeneSodiumReceptor

Abstract

fetched live from OpenAlex

Appearance of highly sodium selective voltage-gated sodium channels with rapid gating kinetics was a limiting factor in the evolution of nervous systems. Two rounds of domain duplications in a basal eukaryote generated a common ion channel template that is shared amongst voltage-gated sodium (Nav1, Nav2) and calcium channels (Cav1, Cav2, Cav3). Conservation of intron splice junctions in the first domain, including a rare U12-type from the minor spliceosome provides support for a shared, distant heritage of sodium and calcium channels. The consequences of a 4x6 transmembrane domain configuration is the emergence of a uniquely asymmetrical domains with a shared architecture. This configuration permits a highly changeable ion selectivity from calcium to sodium ion selectivity by means of a single lysine residue change in the high field strength site of the ion selectivity filter. A non-selective, and slowly-gating Nav2 channel appearing in single cell eukaryotes before the split of animals and fungi, evolved into ten Nav1.x channel genes specialized for vertebrate tissues. A close kinship is evident in the sharing of most intron splice junctions between Nav2 and Nav1 channels. Multicellularity and the appearance of nervous systems was an impetus for Nav1 channels to adapt to high sodium ion selectivity and fast ion gating. Specific nervous system adaptations include a capacity for modulation by protein kinase phosphorylation and tethering elements to protein assemblies in First Initial Segments and nodes of Ranvier. Associated with the novel niches filled by Nav1 channels in metazoans is the convergent evolution of completely differing sets of accessory Navβ subunits in different animal phyla or sub-phyla. Navβ subunits contribute to trafficking and stabilization of Nav1 channels to specialized nerve membrane locales. Navβ subunit isoforms serve as cell adhesion molecules tethering to elements to the extracellular matrix, and contribute to developmental functions such as nerve outgrowth and axon migration.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.076
Threshold uncertainty score0.733

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.206
Teacher spread0.189 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations43
Published2018
Admission routes2
Has abstractyes

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