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Record W2891642771 · doi:10.1111/lam.13072

Stress survival islet 1 contributes to serotype-specific differences in biofilm formation in <i>Listeria monocytogenes</i>

2018· article· en· W2891642771 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueLetters in Applied Microbiology · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicListeria monocytogenes in Food Safety
Canadian institutionsAgriculture and Agri-Food CanadaUniversity of British Columbia
FundersAlberta Innovates Bio SolutionsUniversity of British Columbia
KeywordsBiofilmListeria monocytogenesSerotypeMicrobiologyBiologyGenotypeBacteriaGeneticsGene

Abstract

fetched live from OpenAlex

Listeria monocytogenes has a significant impact on the food industry by forming biofilms on food-processing equipment. Tandem analysis of whole-genome sequencing data with biofilm data from 166 environmental and food-related L. monocytogenes isolates has revealed serotypic and genetic factors that strongly correlate with adherence and biofilm formation, such as lineage, plasmid harbourage, a three-codon deletion in inlA and the presence of the stress survival islet 1 (SSI-1). Strains from serotype 1/2b, the majority of which contained SSI-1, formed the strongest biofilms, while serotype 4b strains, the majority of which did not contain SSI-1, formed the weakest biofilms. When serotype 1/2a was separated by its SSI-1 genotype, SSI-1-positive 1/2a strains demonstrated significantly higher capacity for biofilm formation after 3 days of growth at 30°C (P < 0·0001). Together, these findings indicate that SSI-1 may contribute to serotype-associated differences in the biofilm-forming capacity in L. monocytogenes. SIGNIFICANCE AND IMPACT OF THE STUDY: Parallel analysis of whole-genome sequences and serotype-specific data was performed to identify genetic markers that correlate with increased adherence and biofilm formation in L. monocytogenes. The analyses revealed the hitherto unrecognized role of SSI-1 in biofilm formation, contributing to deeper understanding of genetic factors that influence behaviour of the species in the food processing environment..

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.021
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.042
GPT teacher head0.271
Teacher spread0.229 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it