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Record W2891920145 · doi:10.1161/res.121.suppl_1.336

Abstract 336: Reactive Ion Surface Modification of Vascular Graft Materials Enhances Endothelialization Without Promoting Thrombosis

2017· article· en· W2891920145 on OpenAlexaff
Deirdre E.J. Anderson, Patrick Jurney, Marie F.A. Cutiongco, Grace Pohan, Pascale Chevallier, Diego Mantovani, Evelyn K. F. Yim, Monica T. Hinds

Bibliographic record

VenueCirculation Research · 2017
Typearticle
Languageen
FieldMaterials Science
TopicElectrospun Nanofibers in Biomedical Applications
Canadian institutionsUniversité LavalUniversity of Waterloo
Fundersnot available
KeywordsSurface modificationBiomaterialFibrinPlateletChemistryBaboonPlatelet activationThrombosisX-ray photoelectron spectroscopyEndothelial stem cellBiomedical engineeringBiophysicsIn vitroImmunologyMedicineSurgeryBiochemistryInternal medicineChemical engineeringBiology

Abstract

fetched live from OpenAlex

Previous work demonstrated that poly(vinyl alcohol) (PVA) holds great potential as a cardiovascular biomaterial. PVA prevents thrombosis at least as well as expanded polytetrafluoroethylene (ePTFE) clinical vascular grafts in whole blood studies. However, long term in vivo success of this material will likely depend on its ability to support an endothelial cell (EC) monolayer. To promote EC attachment and growth we are treating the PVA surface with high energy reactive ions. We hypothesize that increasing the concentration of reactive groups on the surface of the PVA will increase endothelial outgrowth cell (EOC, a type of endothelial progenitor cell isolated from whole blood) attachment without increasing thrombosis. To test this hypothesis we exposed PVA films to reactive ion modifications using O 2 , N 2 , and Ar gases at a variety of powers and durations. Treated samples were then characterized with X-ray photoelectron spectroscopy to quantify surface chemistries. Samples were seeded with EOCs to quantify attachment and proliferation using immunohistochemistry. Platelet and fibrin accumulation was measured dynamically in PVA tubes using a baboon arteriovenous shunt with radiolabeled platelets for 1hr. Flow rate was controlled and no anticoagulants were given. O 2 and N 2 treatments encouraged cell adhesion on the PVA films in an energy dependent manner, which correlated to an increase in surface nitrogen. Initial shunt data (n=3) indicate that treated samples did not have increased platelet attachment compared to untreated PVA or ePTFE. In conclusion, reactive ion modified-PVA demonstrates a promising biomaterial by supporting EC growth without increasing thrombosis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.004
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.139
Threshold uncertainty score0.786

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0040.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.001
Scholarly communication0.0000.001
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.096
GPT teacher head0.408
Teacher spread0.312 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2017
Admission routes1
Has abstractyes

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