2064. Rapid Detection of Carbapenmase-Producing <i>Klebsiella pneumoniae</i> (CPK) Directly from Respiratory Secretion and Clinical Characteristics of Patients with CPK from a 1,200-Bed Tertiary Care Hospital in Thailand
Bibliographic record
Abstract
There is an unmet need for rapid detection of carbapenemase-producing Klebsiella pneumoniae (CPK) for patient care and infection control. The BD MAX™ CRE (automated PCR, Geneohm, Canada) test is the fully automated sample-to-result platform for detection of resistance genes, blaKPC, blaOXA-48 and blaNDM within 2 hours. It has been validated for using with colonies and rectal swab. We aimed to evaluate its performance for detection of carbapenemases genes directly from respiratory samples of the patients whom K. pneumoniae (KP) were identified. A total of 169 KP isolates and respiratory samples were collected from patients admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand from June 2017 to December 2017. The automated PCR test was performed directly from respiratory specimens. The results were compared with in-house PCR for detection of carbapenemase genes performed on KP colonies isolated from respiratory specimens as our reference method. Patient and clinical characteristics between patients with CPK and non-CPK were also analyzed. The prevalence of CPK was 10.6% (18/169 isolates). The automated PCR test had 91.12% accuracy, 66.7% sensitivity (95% CI, 40.9–86.6), 94.0% specificity (95% CI, 88.9–86.6), 57.1% positive predictive value (95% CI, 39.5–73.1) and 95.9% negative predictive value (95% CI, 92.48–97.85). Of 18 isolates, blaOXA-48 was the most common carbapenemase gene (17 isolates; 94.4%), followed by blaNDM-1 (7 isolates; 38.9%). A combination of blaOXA-48 and blaNDM-1 was detected in 6 isolates (33.3%). There were 7 (38.8%) colonizations and 11 (61.1%) infections. The significant risk factors for CPK included post-surgery (P = 0.04) and prior antibiotics exposure (P = 0.04). There was a trend toward higher mortality in patients with CPK albeit not significantly (33% vs. 24.5%, P = 0.41). The automated PCR test has an acceptable accuracy with fair sensitivity for the detection of carbapenemase genes. It is unique that OXA-48 and OXA-48/NDM-1 are the most common carbapenemases in our institute. This diagnostic test may be use for rapid diagnosis or infection control purposes. Exposure to antibiotics associated with colonization or infection with CPK. Patients with CPK had higher mortality. All authors: No reported disclosures.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".