The role of transcription factor MYB53 from Arabidopsis thaliana in the regulated production of suberin
Bibliographic record
Abstract
Suberin is a cell wall-associated polymer that is deposited in diverse plant tissues including root exodermis and endodermis, aerial and underground periderms, and seed coats under both normal and stressful conditions.Suberin plays important roles in protecting plants against various stressors but the molecular mechanisms governing the regulated deposition of suberin are currently unclear.I provide evidence here that AtMYB53, AtMYB92, and AtMYB93 of the MYB-type transcription factor family are important regulators of suberin in root endodermis under non-stress conditions.I first characterized an Arabidopsis steroid-inducible line and found that suberin can be rapidly induced in both roots and leaves upon overexpression of MYB53.A suite of suberin biosynthetic genes was positively regulated at the transcriptional level after MYB53 overexpression.I also generated a collection of loss-of-function mutants of MYB53/MYB92/MYB93, which exhibited major reductions of suberin in the endodermis of young roots in comparison to wild-type.The transcripts of all suberin biosynthetic genes tested were down-regulated in the mutants.The identification of master regulators of suberin may provide the means to generate crops that are more stress resistant via enhancement of their suberized cell walls.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".