PSVII-34 The Characterization of Androstenone Binding to Plasma Proteins in Boars.
Bibliographic record
Abstract
Boar taint is an off-odour and off-flavour in heated pork products, resulting from the accumulation of androstenone, a 16-androstene steroid pheromone, in the adipose tissue. Androstenone transport in the plasma is thought to be facilitated by a carrier protein but, this process has yet to be fully characterized. Thus, the objective of this research was to quantify the binding of androstenone in the plasma and characterize the carrier proteins responsible for androstenone transport. Plasma from terminal cross [Duroc (Yorkshire x Landrace)] boars was incubated with radiolabeled [3H]-androstenone with and without the presence of excess unlabeled androstenone, or competitors 3α-androstenol, 3β-androstenol and dehydroepiandrosterone (n=8). Incubations were analyzed by gel filtration high performance liquid chromatography followed by radioisotope detection and the data was assessed in SAS using a one way ANOVA. Radiolabeled androstenone was observed bound primarily to a carrier protein that eluted at approximately 22 minutes, and corresponded to the most abundant protein in the plasma sample, as determined by UV detection. However, androstenone also appeared to bind to a protein that eluted at approximately 21 minutes, but to a much lesser extent. In the presence of unlabeled androstenone, approximately 37.2 ± 2.3% of [3H]-androstenone was displaced from the plasma protein and was not significantly different from the displacement caused by competitors (P = 0.22). This indicated that androstenone binds non-specifically to carrier proteins in the plasma. The molecular weight of the primary protein of interest was determined with gel electrophoresis, and was between 50 and 75 kDa. Furthermore, the protein that eluted at 21 minutes had an approximate molecular weight of 150 kDa. These results provide insight on the way in which the transport of androstenone is facilitated in boars, and with additional research, will aid in the identification of carrier proteins responsible for androstenone transport.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".