Abstract P223: Identification of Chromosome 2 Differentially Expressed Genes Linked to Vascular Inflammation Using Congenic Rats Fed a Normal and High-Salt Diet
Bibliographic record
Abstract
Background: The immune system plays an important role in hypertension. Chromosome 2 (Chr2) introgression from normotensive Brown Norway (BN) rats into hypertensive Dahl salt sensitive (SS) background (consomic SB2) reduced blood pressure (BP), and vascular inflammation under a normal-salt diet (NSD). We hypothesized that BN Chr2 contains anti-inflammatory genes that could prevent BP elevation and vascular inflammation in rats fed NSD and high-salt diet (HSD). These genes will be identified using microRNA (miRNA) and total RNA sequencing in aorta of congenic rats containing different portions of BN Chr2 under NSD and HSD. Methods and Results: Four-to-6 week-old male SS and congenic SB2a and SB2b rats were fed a NSD or HSD (4% NaCl) for 8 weeks. Telemetry systolic BP (SBP) was lower in SB2a and SB2b compared to SS (125±3, 127±6 vs 146±2 mm Hg, P <0.05) under NSD and tended to be higher in SB2b but not in SB2a when compared to SS (185±8, 167±7 vs 168±5 mm Hg) under HSD. RNA was extracted from aorta and used for small and total RNA sequencing using Illumina HiSeq-2500. Differentially expressed (DE) miRNAs and genes (mRNAs and non-coding RNAs [ncRNAs]) encoded in BN Chr2 introgressed portions were identified with fold change ≤ or ≥1.5 and FDR<0.05 in SB2a vs SS (miRNAs: 2 up (↑) and 2 down (↓), mRNAs: 8 ↑ and 10 ↓, ncRNA: 1 ↓) under NSD and (miRNAs: 12 ↑ and 14 ↓; mRNAs: 188↑ and 259 ↓, ncRNAs: 1 ↑ and 11 ↓) under HSD; and SB2b vs SS (miRNAs: 2 ↑ and 4 ↓, mRNAs: 26 ↑ and 85 ↓; ncRNAs: 1 ↑ and 2 ↓) under NSD and (mRNAs: 6 ↑ and 11 ↓, ncRNAs: 1 ↑ and 3 ↓) under HSD. Ddah1 was down-regulated in SB2a vs SS rats fed NSD and HSD, while Acad9 , Agtr1b, ncRNA AABR07012047.1 , Fbxw7, Ptgfrn were down-regulated and ncRNA AABR07012585.3 , Bbs12, Kcnab1 were up-regulated in SB2b vs SS rats fed NSD and HSD. DE RNAs were confirmed by reverse transcription-quantitative PCR (RT-qPCR). Correlation between RNA-sequencing and RT-qPCR data was demonstrated for 6 of 9 tested RNAs: Kcnab1 (r=0.86, P <3.3E-9), Agtr1b (r=0.85, P <6E-9), Ddah1 (r=0.78 , P <5.0E-7) , Bbs12 (r=0.72, P <6.4E-6), Ptgfrn (r=0.54, P <0.005), and AABR07012047.1 (r=0.48, P <0.05). Conclusions: BN Chr2 encoded DE genes were identified in aorta of congenic SB2a and SB2b rats fed NSD and HSD. Whether these genes play a role in vascular inflammation remains to be determined.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".