Complete Genome Amplification and Sequencing of DENV-2 Pakistani Isolates: Analysis Reveals Molecular Evolution of DENV-2
Bibliographic record
Abstract
Aim: This study was aimed to amplify, characterize and analyze complete genomes of dengue virus serotype-2 (DENV-2) Pakistani isolates. Patients & methods: Complete genomes of DENV-2 (n = 4) in nine overlapping fragments from two dengue outbreaks (2011 and 2013) were sequenced; deposited in GenBank (Punjab Isolates: KM217157 and KM217156; Swat Isolates: KM217158 and KJ701507) and characterized using specific primers, we designed. Results: Analysis clustered Pakistani isolates with the cosmopolitan genotypes and further indicated that codons 65 of C, 155 of PrM, 203 of E and 254 of NS1 genes were under negative selection pressure. A substitution of alanine with proline in NS5 gene at nucleotide position 885 in Swat isolates was observed. Conclusion: DENV-2 is spreading to other parts of Pakistan with the possible adaptability of the virus molecularly.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".