Adapting anaerobic consortium to pure and complex lignocellulose substrates at low temperature: kinetics evaluation
Bibliographic record
Abstract
The purpose of this research was to evaluate the kinetics of anaerobic microbial culture during adaptation to pure and complex lignocellulosic substrates at low temperature. Six pairs of 1.0 L batch reactors maintained at 20 °C were fed pure (xylan and cellulose) and complex (cow manure and wheat straw) lignocellulosic substrate in three successive cycles of 35 days each. The biogas volume and composition, chemical oxygen demand, and volatile solids were monitored to evaluate the kinetics of the culture during the adaption. Anaerobic culture adapted to digest the pure and complex lignocellulosic substrates at 20 °C in relatively short period (105 days; 3 successive cycles of 35 days each) in batch reactor studies. The first-order model kinetics revealed that the average increase (day −1 cycle −1 ) in the reaction rate constant over the successive cycles was 0.0831 (xylan) > 0.0235 (cow manure) > 0.0207 (wheat straw) > 0.0123 (xylan:cellulose mixture) > 0.0041 (cellulose). The rates of the substrate degradation at 20 °C were: 0.085–0.093 day −1 (cellulose), 0.112–0.278 day −1 (xylan), 0.112–0.137 day −1 (xylan:cellulose mixture), 0.069–0.116 day −1 (cow manure), and 0.057–0.106 day −1 (wheat straw). Anaerobic mixed culture can be adapted to pure and complex lignocellulosic substrates and convert them to methane at low temperature (20 °C) in relatively short time (105 days) using a sequential procedure. The culture adaptation to wheat straw proceeded at a slower rate than that for cow manure.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".