Expression Analysis of <i>CBL1</i> and <i>CBL6</i> Genes in Sugarcane under Abiotic Stress
Bibliographic record
Abstract
CBLs (Calcineurin B-like proteins) is a type of special calcium sensors in plants, which plays an important role in response to stress. We derived SsCBL1 and SsCBL6 genes in sugarcane from NCBI databases. SsCBL1 gene contained a complete open reading frame of 642 bp, encoding a protein with 213 amino acids. SsCBL6 gene contained a complete open reading frame of 672 bp, encoding a protein with 223 amino acids. The structure of SsCBL1 and SsCBL6 encoding protein was analyzed by bioinformatics methods. The results showed that both of them had no transmembrane segment but contained four EF-hand domains. The phylogenetic tree analysis indicated that SsCBL1 and SsCBL6 were closely related to CBL1 and CBL6 in other plants, which were highly conservative. The results of real-time quantitative PCR demonstrated that the expression of two genes changed under low nitrogen, low phosphorus, low potassium, drought, salt and ABA stress, respectively. The expression was strongly induced by drought and salt treatments for SsCBL1 , while the expression of SsCBL6 was strongly induced by low potassium, drought and salt treatment. Real-time quantitative analysis suggested that SsCBL1 and SsCBL6 might play important regulatory roles under various stresses.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".