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Record W2909310706 · doi:10.1172/jci123959

Loss of the sphingolipid desaturase DEGS1 causes hypomyelinating leukodystrophy

2019· article· en· W2909310706 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueJournal of Clinical Investigation · 2019
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSphingolipid Metabolism and Signaling
Canadian institutionsMcMaster Children's Hospital
FundersEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNational Center for Advancing Translational SciencesNational Institute of Neurological Disorders and StrokeNational Human Genome Research InstituteEuropean Regional Development FundAgència de Gestió d'Ajuts Universitaris i de RecercaCanadian Institutes of Health ResearchU.S. NavyIntellectual and Developmental Disabilities Research CenterNational Institutes of HealthUniversité Paris DiderotMinisterio de Economía y CompetitividadFondation pour la Recherche MédicaleGeneralitat de CatalunyaBaylor-Hopkins Center for Mendelian GenomicsAgence Nationale de la RechercheInstituto de Salud Carlos IIINational Research FoundationNational Multiple Sclerosis SocietyFundación HesperiaCenter for Individualized Medicine, Mayo ClinicCentres de Recerca de CatalunyaOntario Genomics InstituteHoward Hughes Medical InstituteGeorgia Clinical and Translational Science AllianceSimons Foundation Autism Research InitiativeGénome QuébecMayo ClinicNational Ataxia FoundationOntario GenomicsU.S. Department of DefenseSimons FoundationGenome CanadaYale UniversityInstitut National de la Santé et de la Recherche MédicaleJohns Hopkins UniversityMcGill UniversityEuropean CommissionBroad Institute
KeywordsSphingolipidLeukodystrophyMyelinFingolimodBiologySpasticityEndoplasmic reticulumZebrafishNeuroscienceCeramideCell biologyMedicineInternal medicineGeneticsMultiple sclerosisImmunologyDiseaseGenePhysical medicine and rehabilitationCentral nervous system

Abstract

fetched live from OpenAlex

Sphingolipid imbalance is the culprit in a variety of neurological diseases, some affecting the myelin sheath. We have used whole-exome sequencing in patients with undetermined leukoencephalopathies to uncover the endoplasmic reticulum lipid desaturase DEGS1 as the causative gene in 19 patients from 13 unrelated families. Shared features among the cases include severe motor arrest, early nystagmus, dystonia, spasticity, and profound failure to thrive. MRI showed hypomyelination, thinning of the corpus callosum, and progressive thalamic and cerebellar atrophy, suggesting a critical role of DEGS1 in myelin development and maintenance. This enzyme converts dihydroceramide (DhCer) into ceramide (Cer) in the final step of the de novo biosynthesis pathway. We detected a marked increase of the substrate DhCer and DhCer/Cer ratios in patients' fibroblasts and muscle. Further, we used a knockdown approach for disease modeling in Danio rerio, followed by a preclinical test with the first-line treatment for multiple sclerosis, fingolimod (FTY720, Gilenya). The enzymatic inhibition of Cer synthase by fingolimod, 1 step prior to DEGS1 in the pathway, reduced the critical DhCer/Cer imbalance and the severe locomotor disability, increasing the number of myelinating oligodendrocytes in a zebrafish model. These proof-of-concept results pave the way to clinical translation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.003
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.009
Threshold uncertainty score0.340

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.003
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.034
GPT teacher head0.320
Teacher spread0.286 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it