Cover Feature: A Bioluminescent Ca<sup>2+</sup> Indicator Based on a Topological Variant of GCaMP6s (ChemBioChem 4/2019)
Bibliographic record
Abstract
The cover feature picture shows how a change in the topology of an optimized genetically encoded Ca2+ indicator, GCaMP6s, led to a variant that retains the favorable properties of the original but also has new termini in closer proximity to the chromophore. Fusion of a bright luciferase to GCaMP6s (with the original topology) does not generate high bioluminescence resonance energy transfer (BRET) efficiency between the donor and acceptor either in the presence or absence of Ca2+. However, fusion of bright luciferase to the topological variant of GCaMP6s results in high BRET efficiency in the presence of Ca2+ and low BRET efficiency in its absence. This fusion protein is thus a highly effective glow-in-the-dark Ca2+ indicator. More information can be found in the communication by R. E. Campbell et al. on page 516 in Issue 4, 2019 (DOI: 10.1002/cbic.201800255). Image by Y.Q.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".