Cover Feature: A Bioluminescent Ca<sup>2+</sup> Indicator Based on a Topological Variant of GCaMP6s (ChemBioChem 4/2019)
Bibliographic record
Abstract
The cover feature picture shows how a change in the topology of an optimized genetically encoded Ca2+ indicator, GCaMP6s, led to a variant that retains the favorable properties of the original but also has new termini in closer proximity to the chromophore. Fusion of a bright luciferase to GCaMP6s (with the original topology) does not generate high bioluminescence resonance energy transfer (BRET) efficiency between the donor and acceptor either in the presence or absence of Ca2+. However, fusion of bright luciferase to the topological variant of GCaMP6s results in high BRET efficiency in the presence of Ca2+ and low BRET efficiency in its absence. This fusion protein is thus a highly effective glow-in-the-dark Ca2+ indicator. More information can be found in the communication by R. E. Campbell et al. on page 516 in Issue 4, 2019 (DOI: 10.1002/cbic.201800255). Image by Y.Q.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.320 | 0.083 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".