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Record W2914370832 · doi:10.1139/cjz-2018-0230

Oxidative stress does not differ in primary dermal fibroblasts isolated from fast-growing and control-growing Japanese Quail (<i>Coturnix japonica</i>)

2019· article· en· W2914370832 on OpenAlexvenueno aff
Ursula K. Beattie, Ana Gabriela Jiménez

Bibliographic record

VenueCanadian Journal of Zoology · 2019
Typearticle
Languageen
FieldEnvironmental Science
TopicPhysiological and biochemical adaptations
Canadian institutionsnot available
Fundersnot available
KeywordsQuailBiologyOxidative stressCoturnix coturnixCoturnixReactive oxygen speciesLipid peroxidationGlutathioneJaponicaMitochondrial ROSDNA damageOxidative damageCell biologyBiochemistryBotanyEndocrinologyDNA

Abstract

fetched live from OpenAlex

Growth rate is a key life-history trait that influences fitness and shapes the physiology of organisms. Additionally, faster growing individuals of the same species seem to be burdened with higher whole-animal metabolism and higher cellular turnover rates, which may lead to increases in oxidative stress, though this fact remains controversial within the literature. Aerobic organisms are subjected to metabolic by-products known as reactive oxygen species (ROS), which can wreak havoc on macromolecules, such as structurally altering proteins and inducing mutations in DNA, among others. To combat accumulating damage, organisms have evolved endogenous antioxidants and can consume exogenous antioxidants to sequester ROS before they cause cellular damage. We used primary fibroblast cells isolated from control-growing and fast-growing Japanese Quail (Coturnix japonica Temminck and Schlegel, 1849) as a study model for the effects of differing growth rates on oxidative stress. We measured reduced glutathione (GSH) concentration, ROS production, mitochondrial content, and lipid peroxidation (LPO) damage. We found no significant differences in the four parameters measured between control-growing and fast-growing Quail. However, we found that in fast-growing Quail, GSH correlated with LPO damage and mitochondrial content, and LPO damage positively correlated with mitochondrial content, whereas control-growing Quail only showed positive relationships between LPO damage and ROS production.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.182
Teacher spread0.177 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2019
Admission routes1
Has abstractyes

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Same venueCanadian Journal of Zoology→Same topicPhysiological and biochemical adaptations→French-language works237,207→