Analysis of Key Enzyme Genes in Carotenoid Metabolism Pathway of <i>Lilium</i> and Cloning of <i>LoLcyB</i>
Bibliographic record
Abstract
In this study, five growth stages of Oriental lily ‘Justina’ leaves (10 d, 25 d, 50 d, 75 d and 100 d), and seven different parts of styles, anthers, petals, leaves, stems, bulbs and roots in 100 day growth were used as test materials to measure the content of carotenoid and chlorophyll in Lilium by organic solvent method. The semi-quantitative expression analysis of 12 key enzyme genes ( PSY , PDS , Z-ISO , ZDS , CRTISO , LCYB , LCYE , HYB , VDE , ZEP , CCS and NCED ) of Lilium carotenoid metabolic pathway was carried out according to the sequencing results of transcriptome library. The study cloned the open reading frame (ORF) sequence of Lilium LCYB gene and carried out related bioinformatics analysis. The results showed that carotenoid and chlorophyll content were significantly different in the five different stages of Lilium leaf growth and seven different parts of Lilium ripening stage. The expression levels of 12 key enzyme genes were variant in different growth stages and parts. The complete ORF of the LCYB gene was cloned with the whole length of 1,503 bp, encoding 500 amino acids, which had typical conserved region characteristics of Carotene Cycl super family. It showed the closest relationship with Musa acuminata among the known genome-wide plants. This study might provide a solid theoretical basis for further study of Lilium carotenoid metabolic pathways and further understanding of the molecular mechanism of Lilium color and leaf color synthesis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".