NLRC5 deficiency has a moderate impact on immunodominant <scp>CD</scp>8<sup>+</sup> T‐cell responses during rotavirus infection of adult mice
Bibliographic record
Abstract
Abstract The NOD ‐like receptor ( NLR ) family plays an important role in innate immunity. Class II transactivator and NOD‐like receptor caspase activation and recruitment domain CARD containing 5 (NLRC5) are unusual members of the NLR family that instead of recognizing pathogen‐associated or damage‐associated molecular patterns, form enhanceosomes with adaptor molecules and modulate major histocompatibility complex ( MHC ) class II and MHC class I expression, respectively. While NLRC 5 has been shown to play a role during intracellular pathogen infection and tumor cell immune evasion, its role in regulating antigen‐specific CD 8 + T‐cell responses at the intestinal mucosa has not been investigated. Here, we take advantage of the rotavirus model in adult mice to dissect the impact of NLRC 5 on CD 8 + T‐cell responses to this viral infection at the gut mucosa. We show that while Nlrc5 −/− mice exhibited normal proportions of T‐cell subpopulations in the intraepithelial and lamina propria compartments, these mice had decreased baseline MHC class I expression on various immune cells in the lamina propria. Upon rotavirus infection, Nlrc5 deficiency resulted in impaired H2‐K b ‐restricted antigen‐specific CD 8 + T‐cell responses, which were recapitulated in mice deficient for Nlrc5 within the dendritic cell compartment. The impaired CD 8 + T‐cell response in Nlrc5 −/− mice was not significant enough to impact viral titers, suggesting compensation in Nlrc5 −/− mice, perhaps as a result of higher numbers of activated B cells in the mesenteric lymph nodes and normal rotavirus‐specific immunoglobulin A responses. Collectively, our results demonstrate a minor role for NLRC 5 in modulating H2‐K b ‐restricted antigen‐specific CD 8 + T‐cell responses in the small intestine during rotavirus infection in adult mice.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".