A287 CARACTÉRISATION DE LA SIGNALISATION P2Y 6 DANS LA MIGRATION CELLULAIRE .
Bibliographic record
Abstract
Colorectal cancer (CRC) is the third most prevalent form of cancer worldwide and the second most diagnosed form of cancer in Canada. G protein-coupled receptors (GPCRs) are suspected to play a key role in CRC tumorigenesis. The tumorigenic process is divided into three steps: initiation, promotion and tumor progression. Formation of metastases is based on cell cancer capacity to migrate during early stages of tumorigenesis. Identification of new targets involved in CRC development is a major challenge to improve diagnostic and treatment of this form of cancer. Past research has identified the GPCR P2Y6 as such innovative target. In fact, P2Y6 activities were involved in different stages of colorectal tumorigenesis. We demonstrated that P2y6-/- mice significantly develop fewer tumors than control mice in a CRC mouse model. Other studies confirmed that P2Y6 played a role in the epithelial to mesenchymal transition (EMT) and accelerated migration of breast cancer MDA-MB-468 cells. Surprisingly, there is sparse information about the potential influence of P2Y6 action in cancer cell migration and on the mechanism involved in this process. That’s why, in this study, we wanted to characterise P2Y6 signalling in cell migration. We studied effects of this receptor on cell migration by using wound healing assays and we showed migrating structures by immunofluorescence assays and western blots. In this work, we showed that P2Y6 activation lead to cell migration using wound healing assays. This migratory stimulating effect was the result of the formation of filopodia and focal adhesions, known as migrating structures, and the cofilin phosphorylation. The formation of filopodia was associated to the P2Y6 dependent modulation of CDC42 protein expression whereas cofilin phosphorylation was correlated to the Rho/ROCK pathway. These results are thus supporting the idea that the P2Y6 receptor is involved in cell migration and it could be a target for the treatment of CRC. NoneFinancement par mes directeurs le Pr. Fernand-Pierre Gendron (50%) et le Pr. Philippe Sarret (50%).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".