A161 INFECTION WITH THE TAPEWORM HYMENOLEPIS DIMINUTA EVOKES A MASSIVE, BUT DELAYED INCREASE IN TUFT CELLS IN THE MURINE INTESTINE
Bibliographic record
Abstract
The intestinal epithelium is composed of multiple cell types that work in harmony towards digestion, absorption, defence and repair. The enteric tuft cell is a chemosensory cell that produces IL-25 as an early signal following infection with nematode (roundworm) parasites to activate innate lymphoid cells (ILC2) and engage Th2 immunity. Despite rapid expulsion from the non-permissive mouse host, the cestode, H. diminuta is non-invasive and causes no damage to the gut. It is unknown if tuft cell activation is a component of the host response to enteric infection with flatworm parasites, and H. diminuta in particular. To assess changes in enteric tuft cells following infection with the non-invasive, non-damaging tapeworm, H. diminuta. BALB/c mice were infected with 5 H. diminuta cysticercoids and cryostat sections of mid- jejunum, ileum and colon were studied at 5, 8 and 11 days post-infection. Time-matched, non-infected mice served as controls. Sections were immunostained using antibodies against doublecortin-like kinase -1 (DCLK-1), a tuft cell-specific marker, and blindly scored for tuft cell number. DCLK-1+ cells in the epithelium were readily detectable in the gut of non-infected and infected mice. Sections of the intestine appeared histologically normal at all time points. Intestine from 5 and 8 days post-infection showed no change in tuft cell number compared to non-infected mice. However, a dramatic tuft cell hyperplasia (~10-fold increase) in jejunal, ileal and colonic sections was apparent at 11 days post-infection (nb. H. diminuta is a parasite of the small intestine), by which time the worm has been expelled from the gut. There was a noticeable aggregation of tuft cells around sections of Peyer’s patches in the ileum from infected mice. Tuft cell hyperplasia along the gut accompanies the later stages of infection with H. diminuta, that may promote a generalized Th2 environment during and post-infection. Factors (e.g. IL4Rα) responsible for the tuft cell response, any tuft cell heterogeneity, and a role for tuft cells in worm expulsion need to be addressed. CIHRNSERC
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".