A59 EXPLORING THE ROLE OF MICROBIOTA IN GASTROINTESTINAL MOTILITY
Bibliographic record
Abstract
Interactions between microbiota and gastrointestinal (GI) function may be evident from the early stage of life. Microbiota contributes to myenteric nerve development and normal gut function maintenance. Earlier studies suggested that germ-free or antibiotics treated mice have decreased gastrointestinal transit. Also, abnormal gut microbiota is common in patients with altered GI motility, like IBS. However, the underlying mechanism are not fully understood. Here, we investigated the role of microbiota in GI motility in vivo and vitro. Specific-pathogen-free (SPF) mice and germ-free (GF) mice (12–16 weeks old) were used. Gastric emptying was assessed by videofluoroscopy and GI transit was assessed by SHAPE study using metallic beads, as described previously. Mice were then sacrificed and samples from jejunum, ileum and mid-colon were taken. Isometric contraction was assessed after electric field stimulation (EFS) and pharmacological treatments. Acetylcholine (Ach) release was assessed by superfusion using isotope [3H]. Expression of genes related to neuromuscular function was assessed by Illumina, immunostaining was also performed. There were no significant difference in gastric emptying rates between SPF and GF mice, which were 26.4±4.1 % (n=10) and 23.1±3.1 % (n=11), respectively. However, GI transit (total scores) was faster in SPF mice compared to GF mice, with 13.6±1.7 (n=10) and 5.4±0.2 (n=11) respectively. Beads were mainly located in the cecum in SPF mice, but in GF mice, most of them remained in the proximal small intestine. In contractility studies, carbachol induced similar level of contraction in SPF or GF tissues, both in the small bowel and colon. In SPF tissues, the neural stimulation caused a transient contraction in jejunum, and biphasic relaxation and contraction in ileum and colon. In GF tissues, neural stimulation caused similar response in ileum and colon, however, in jejunum, it caused biphasic response with a significant relaxation followed by a contraction. No difference was seen in EFS-induced Ach release between SPF and GF tissues. Microbiota appears to be essential in the establishment of normal small intestinal motility. Germ-free mice have altered jejunal motility, likely due to a non-cholinergic neural response. Gene expression study and immunostaining are underway to reveal the underlying mechanism. None
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".