528 Does Split Thickness Graft Grow from bottom up in Large Burn Injury?
Bibliographic record
Abstract
It is well established that autologous split thickness skin graft is necessary for the survival of patients with large burns and skin defects. As in patients, our preliminary study in pig showed that a thin partial thickness graft became remarkable thicker within 20 days. Here, we have asked the question of whether a partial thickness skin graft grows from bottom up in a large skin injury such as burn. To answer this question, we hypothesized that growth of split thickness graft should be from bottom up through conversion of immune cells into collagen producing skin cells. In this study, we have used a preclinical Yorkshire pig as a model whose skin thickness and adherence to the underlying structures are similar to those of human skin. The partial thickness graft at 0.015 inch/ 0.38 mm was harvested by an electrical dermatome and meshed at 3:1 ratio and grafted on 50 x 50 x 3 mm full thickness wound in pigs. New tissue formation was evaluated in 8 mm in diameter punches taken on day 10 and 20 post-operation. Tissue samples were processed, sectioned and co-stained for immune cell markers (CD45) and type I collagen. The finding revealed that a partial thickness graft grew from bottom up and reached to almost the same level as uninjured skin within 60 days post operation. Further analysis revealed that more than 7,000 cubic mm new tissue was formed from the bottom up of large full thickness wounds in pig receiving partial thickness (0.38 mm) meshed grafts. The result of immuno- staining identified a large number of cells, which co-expressed immune cell marker (CD45) and collagen on day 10 post operation. Interestingly, as the number of these cells reduced on day 20, most of these cells became positive for collagen production. The early thickening of partial thickness graft is likely happening through a major contribution of infiltrated immune cells that convert into mainly collagen producing fibroblasts in large burn injuries. Identifying the factors responsible for conversion of immune cells to skin cells would make it possible to topically apply these factors to promote the healing and reduce inflammation in large burn injury.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".