74 Adipose Tissue from Burn Patients is Proinflammatory, Lipolytic and a Key Initiator of Hepatic Dysfunction
Bibliographic record
Abstract
Severe burns induce systemic dysfunction characterized by chronic increased resting energy expenditure, catabolism of fat and muscle tissues, hepatomegaly and liver damage. The role of adipose tissue in initiating these complications has not been adequately defined. The aims of this study were to characterize the adipose tissue secretome of burn patients and delineate the extent of damage these biomolecules exert on hepatocytes compared to healthy control adipose. White adipose tissue (WAT) from burn patients admitted to a local burn centre requiring surgery and healthy controls undergoing elective procedures were used to prepare explant cultures. Gas chromatography-mass spectrometry and cytokine arrays were applied to characterize samples. These were subsequently incubated with human hepatocytes (HepG2) to elucidate their effects on cellular function. Seahorse XF96 analysis was used to study mitochondrial dynamics, and expression of endoplasmic reticulum (ER) stress and apoptosis markers were probed. Here, we show that WAT from burn patients undergoes extensive lipolysis and the release of inflammatory mediators compared to healthy control tissue. Treatment of hepatocytes with burned adipose explant media significantly raised ER stress and apoptosis markers while increasing mitochondrial oxygen uptake and inducing UCP2-mediated uncoupling (p ≤ 0.05). Further, the adipose secretome of burn patients alters nutrient homeostasis and mitophagy in hepatocytes, as indicated by the phosphorylation of acetyl-CoA carboxylase (Ser 79) and changes to mTORC1 and PINK1 expression. The adipose tissue, generally regarded as a mere storage depot, is often overlooked as a contributor to systemic dysfunction post-trauma. Our results single out the adipose secretome of burn patients as an inducer of hepatocellular ER stress, mitochondrial hyperfusion/uncoupling and ultimately, cell death. The data herein indicate that the adipose tissue acts as an initiator of systemic alterations following burn. Therapeutic interventions which reverse these morphological changes to WAT may be beneficial in reducing systemic complications.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".