Cover Feature: Influence of Linker Length on Ligase‐Catalyzed Oligonucleotide Polymerization (ChemBioChem 6/2019)
Bibliographic record
Abstract
The cover feature picture shows Goldilocks offering a pentanucleotide (flower) to the middle-sized Mama bear who holds the ideal linker length to display the R group modification. In our work, we explored the effects of decreased linker lengths on the efficiency, fidelity, and bias of Ligase-catalyzed OligOnucleotide PolymERizations (LOOPER). Whereas longer linker lengths can have a negative impact on binding thermodynamics, shorter linker lengths result in high error and bias during LOOPER. An ethane-1,2-diamine linker was found to be optimal in all respects, and should facilitate the discovery of highly modified aptamer isolated from in vitro evolution of LOOPER-derived DNA libraries. More information can be found in the full paper by R. Hili et al. on page 793 in Issue 6, 2019 (DOI: 10.1002/cbic.201800616).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.279 | 0.065 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".