Cover Feature: Influence of Linker Length on Ligase‐Catalyzed Oligonucleotide Polymerization (ChemBioChem 6/2019)
Bibliographic record
Abstract
The cover feature picture shows Goldilocks offering a pentanucleotide (flower) to the middle-sized Mama bear who holds the ideal linker length to display the R group modification. In our work, we explored the effects of decreased linker lengths on the efficiency, fidelity, and bias of Ligase-catalyzed OligOnucleotide PolymERizations (LOOPER). Whereas longer linker lengths can have a negative impact on binding thermodynamics, shorter linker lengths result in high error and bias during LOOPER. An ethane-1,2-diamine linker was found to be optimal in all respects, and should facilitate the discovery of highly modified aptamer isolated from in vitro evolution of LOOPER-derived DNA libraries. More information can be found in the full paper by R. Hili et al. on page 793 in Issue 6, 2019 (DOI: 10.1002/cbic.201800616).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".