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Record W2926181828 · doi:10.1101/594457

SNPs associated with <i>HHIP</i> expression have differential effects on lung function in males and females

2019· preprint· en· W2926181828 on OpenAlexaff
Katherine A. Fawcett, Ma’en Obeidat, CA Melbourne, Nick Shrine, AL Guyatt, Catherine John, Jian’an Luan, Anne Richmond, M. Moksnes, Raquel Granell, Medea Imboden, Sebastian May-Wilson, Pirro G. Hysi, TS Boutin, Laura Portas, Claudia Flexeder, SE Harris, CA Wang, Leo‐Pekka Lyytikäinen, Teemu Palviainen, Rachel E. Foong, Dirk Keidel, Cosetta Minelli, Claudia Langenberg, Yohan Bossé, Berge M van den, Don D. Sin, Ke Hao, Archie Campbell, David J. Porteous, Sandosh Padmanabhan, BH Smith, David M. Evans, Susan M. Ring, Arnulf Langhammer, Kristian Hveem, Cristen J. Willer, Ralf Ewert, Beate Stubbe, Nicola Pirastu, Lucija Klarić, PK Joshi, Karina Patasova, Mangino Massimo, Ozren Polašek, JM Starr, Igor Rudan, Taina Rantanen, Kirsi H. Pietiläinen, Mika Kähönen, OT Raitakari, Graham L. Hall, Peter D. Sly, Craig E. Pennell, Jaakko Kaprio, Terho Lehtimäki, Véronique Vitart, Ian J. Deary, Deborah Jarvis, J.F. Wilson, Timothy Spector, Nicole Probst‐Hensch, Nicholas J. Wareham, Henry Völzke, A. John Henderson, David P. Strachan, Ben Brumpton, Caroline Hayward, Ian P. Hall, Martin D. Tobin, LV Wain

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2019
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEpigenetics and DNA Methylation
Canadian institutionsInstitut universitaire de cardiologie et de pneumologie de QuébecCentre for Global Health ResearchSt. Paul's HospitalUniversity of British Columbia
FundersNIHR Nottingham Biomedical Research CentreNIHR Leicester Biomedical Research CentreBiotechnology and Biological Sciences Research CouncilBritish Lung FoundationMedical Research CouncilNational Institute for Health and Care ResearchWellcome Trust
KeywordsSingle-nucleotide polymorphismLung cancerLungLung functionSpirometryBiologySNPGenotypeInternal medicinePhysiologyOncologyMedicineGeneticsGene

Abstract

fetched live from OpenAlex

Abstract Adult lung function is highly heritable and 279 genetic loci were recently reported as associated with spirometry-based measures of lung function. Though lung development and function differ between males and females throughout life, there has been no genome-wide study to identify genetic variants with differential effects on lung function in males and females. Here, we present the first genome-wide genotype-by-sex interaction study on four lung function traits in 303,612 participants from the UK Biobank. We detected five SNPs showing genome-wide significant (P<5 × 10 −8 ) interactions with sex on lung function, as well as 21 suggestively significant interactions (P<1 × 10 −6 ). The strongest sex interaction signal came from rs7697189 at 4:145436894 on forced expiratory volume in 1 second (FEV 1 ) (P = 3.15 × 10 −15 ), and was replicated (P = 0.016) in 75,696 individuals in the SpiroMeta consortium. Sex-stratified analyses demonstrated that the minor (C) allele of rs7697189 increased lung function to a greater extent in males than females (untransformed FEV 1 β = 0.028 [SE 0.0022] litres in males vs β = 0.009 [SE 0.0014] litres in females), and this effect was not accounted for by differential effects on height, smoking or age at puberty. This SNP resides upstream of the gene encoding hedgehog-interacting protein ( HHIP ) and has previously been reported for association with lung function and HHIP expression in lung tissue. In our analyses, while HHIP expression in lung tissue was significantly different between the sexes with females having higher expression (most significant probeset P=6.90 × 10 −6 ) after adjusting for age and smoking, rs7697189 did not demonstrate sex differential effects on expression. Establishing the mechanism by which HHIP SNPs have different effects on lung function in males and females will be important for our understanding of lung health and diseases, such as chronic obstructive pulmonary disease (COPD), in both sexes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0050.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.215
Teacher spread0.205 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2019
Admission routes1
Has abstractyes

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