SAT-347 Unfolding Generates an Active CYP11A1 for Mitochondrial Integration and Activity
Bibliographic record
Abstract
Mitochondrial protein import and sorting are mediated by translocases. Precursor proteins with amino-terminal presequences transition from the translocase of the outer membrane (TOM) complex to the translocase of the inner membrane (TIM) complex. In adrenal and gonadal cells, the molecular mechanism of mitochondrial complex formation and electron transport to initiate and maintain steroid metabolic activity has remained unclear. On the mitochondrial surface, Tom22 or Tom20 directs precursor import through the pore-forming subunit, Tom40. After emerging from the Tom40 channel, presequence-containing precursors engage with the presequence translocase of the inner membrane or reach the matrix directly as a permanent resident or are integrated into the membrane. Thus, the activity of the inner membrane translocase is tightly regulated by targeting signals in a yet undefined manner. For the first time, we show that testicular as well as adrenal mitochondrial metabolic activity is dependent on more than one protein, where Tim50 and SCC are equally important, and depends on the specific unfolding conformation necessary for SCC. Following mitochondrial import of SCC, the N-terminus is first cleaved rapidly and then slowly cleaved a second time. The unfolded protein is transported to the IMS, where the C-terminus is inserted into the TIM23 complex, resulting in a significantly unfolded protein to interact and associate with Tim50 for metabolic activity. Therefore, Tim50 is possibly playing a role of a key factor for SCC metabolic activity in addition to the co-factors ferrodoxin and ferrodoxin reductase.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".