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Record W2951905878 · doi:10.1038/s41467-018-08246-y

Hydrogen-based metabolism as an ancestral trait in lineages sibling to the Cyanobacteria

2019· article· en· W2951905878 on OpenAlexfundno aff
Paula B. Matheus Carnevali, Frederik Schulz, Cindy J. Castelle, Rose S. Kantor, Patrick M. Shih, Itai Sharon, Joanne M. Santini, Matthew R. Olm, Yuki Amano, Brian C. Thomas, Karthik Anantharaman, David Burstein, Eric D. Becraft, Ramūnas Stepanauskas, Tanja Woyke, Jillian F. Banfield

Bibliographic record

VenueNature Communications · 2019
Typearticle
Languageen
FieldEnvironmental Science
TopicMicrobial Community Ecology and Physiology
Canadian institutionsnot available
FundersLawrence Berkeley National LaboratoryBiological and Environmental ResearchJapan Atomic Energy AgencyOffice of ScienceGrand Challenges CanadaJoint Genome InstituteAustralian GovernmentU.S. Department of EnergyUniversity of SaskatchewanNational Science Foundation
KeywordsCyanobacteriaCellular respirationBiologyAnaerobic respirationNitrogenaseGenomeFermentationPhotosynthesisMetabolismGeneElectron transport chainBiochemistryRespirationGeneticsBacteriaBotanyNitrogen fixation

Abstract

fetched live from OpenAlex

Abstract The evolution of aerobic respiration was likely linked to the origins of oxygenic Cyanobacteria. Close phylogenetic neighbors to Cyanobacteria, such as Margulisbacteria (RBX-1 and ZB3), Saganbacteria (WOR-1), Melainabacteria and Sericytochromatia, may constrain the metabolic platform in which aerobic respiration arose. Here, we analyze genomic sequences and predict that sediment-associated Margulisbacteria have a fermentation-based metabolism featuring a variety of hydrogenases, a streamlined nitrogenase, and electron bifurcating complexes involved in cycling of reducing equivalents. The genomes of ocean-associated Margulisbacteria encode an electron transport chain that may support aerobic growth. Some Saganbacteria genomes encode various hydrogenases, and others may be able to use O 2 under certain conditions via a putative novel type of heme copper O 2 reductase. Similarly, Melainabacteria have diverse energy metabolisms and are capable of fermentation and aerobic or anaerobic respiration. The ancestor of all these groups may have been an anaerobe in which fermentation and H 2 metabolism were central metabolic features. The ability to use O 2 as a terminal electron acceptor must have been subsequently acquired by these lineages.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.808
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0020.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.297
Teacher spread0.280 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations99
Published2019
Admission routes1
Has abstractyes

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