Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance, and total tract digestibility of heifers fed a high forage diet1
Bibliographic record
Abstract
A metabolism study was conducted using 8 ruminal cannulated beef heifers to investigate the effects of a recombinant fibrolytic enzyme (RFE; xylanase XYL10C) selected specifically for forage-fed ruminants on ruminal pH, fermentation, nitrogen balance, and total tract digestibility of heifers. The experiment was a cross-over design with 2 treatments and 2 periods. The 2 treatments were a basal diet containing 60% barley silage, 30% barley straw, and 10% supplement (DM basis) without (control) or with RFE. The enzyme was sprayed onto the barley straw at a rate of 6.6 × 104 IU·kg-1 DM 24 h before feeding. Each period comprised 2 wk of diet adaptation and 1 wk of sampling and data collection. Feed intake and total tract digestibility of DM, OM, NDF, and ADF were unaffected by RFE. Ruminal pH including mean, minimum, maximum, and duration pH <5.8, did not differ between treatments. Total VFA concentration, molar proportion of individual VFA, and acetate-to-propionate ratio were also not affected by RFE. However, ruminal NH3-N concentration (P < 0.06) and endoglucanase activity (P < 0.08) in ruminal fluid tended to be higher with RFE. Nitrogen utilization and microbial protein synthesis were not affected by treatment. These results indicate that XYL10C did not improve fiber digestion in heifers fed a high forage diet, despite the fact that it was specifically selected for this trait in laboratory assays. However, the increased ruminal NH3-N concentration suggests it potentially increased ruminal proteolytic activity.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".