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Record W2954543961 · doi:10.1002/naaq.10096

Effects of Cecropin Transgenesis and Interspecific Hybridization on the Resistance to <i>Ichthyophthirius multifiliis</i> in Channel Catfish and Female Channel Catfish × Male Blue Catfish Hybrids

2019· article· en· W2954543961 on OpenAlexaff
Ahmed Elaswad, Karim Khalil, Zhi Ye, Ahmed Saud Alsaqufi, Hisham A. Abdelrahman, Baofeng Su, Dayan A. Perera, Sheng Dong, Nermeen Y. Abass, Rex A. Dunham

Bibliographic record

VenueNorth American Journal of Aquaculture · 2019
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAnimal Genetics and Reproduction
Canadian institutionsMinistry of Agriculture
FundersU.S. Department of Agriculture
KeywordsCatfishIctalurusBiologyCecropinIchthyophthirius multifiliisTransgeneFisheryMicrobiologyGeneticsGeneAntimicrobial peptidesFish <Actinopterygii>

Abstract

fetched live from OpenAlex

Abstract The resistance of Channel Catfish Ictalurus punctatus, hybrid catfish (female Channel Catfish × male Blue Catfish I. furcatus [CB hybrids]), and Channel Catfish and hybrid catfish expressing the introduced cecropin B gene to Ichthyophthirius multifiliis infestation was investigated in two experiments. In experiment I, four fingerling groups were challenged, including cecropin-transgenic Channel Catfish, cecropin-transgenic CB hybrids, non-transgenic Channel Catfish, and non-transgenic CB hybrids. Non-transgenic Channel Catfish survived for a shorter time than the other three groups. Survival rate was significantly different between non-transgenic Channel Catfish and the other groups, which had similar survival rates. In experiment II, non-transgenic CB hybrids had a less severe infestation than non-transgenic Channel Catfish. Mortality rates were 62.4% and 40.2% for non-transgenic Channel Catfish and CB hybrids, respectively. The mean survival time for non-transgenic hybrids was significantly longer (&amp;gt;5 d) than that of non-transgenic Channel Catfish. The results suggest that genetic enhancement of Ichthyophthirius resistance can be accomplished in Channel Catfish by either cecropin transgenesis or interspecific hybridization. In addition to survival rate, improving survival time is important because the extension of survival time provides greater opportunity to apply treatments to stop the protozoan infestation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.268
Threshold uncertainty score0.621

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.204
Teacher spread0.199 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations14
Published2019
Admission routes1
Has abstractyes

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