Unveiling subgroups of 16SrIII phytoplasmas affecting Trillium sp. and Melothria pendula in Brazil and Canada
Bibliographic record
Abstract
Melothria pendula and Trillium spp. are important weed species in Brazil and Canada. M. pendula plants showing symptoms of chlorosis, little leaf and leaf malformation and T. grandiflorum and T. erectum exhibiting virescence were tested for phytoplasma presence by nested PCR with primers that amplify the 16S rRNA, ribosomal protein, and secA genes, and subjected to RFLP and sequencing analyses. A phytoplasma of group 16SrIII was identified from M. pendula and classified as a new variant of subgroup 16SrIII-U. The 16SrIII phytoplasmas, first reported in T. grandiflorum and T. erectum in Ontario in 2016 were classified as members of the subgroup 16SrIII-F. Besides, one strain from each Trillium species examined, corresponded to a new variant of subgroup 16SrIII-F (for T. grandiflorum) and a new variant of subgroup 16SrIII-J (for T. erectum). Results refer to the first report of a phytoplasma affecting M. pendula, and the identification of new variants of 16SrIII phytoplasma subgroups in Melothria and Trillium species, which may pose a threat for the spread of 16SrIII phytoplasmas to other plant species grown nearby.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".