p116<sup>Rip</sup> promotes myosin phosphatase activity in airway smooth muscle cells
Bibliographic record
Abstract
Abstract Myosin phosphatase‐Rho interacting protein (p116 Rip ) was originally found as a RhoA‐binding protein. Subsequent studies by us and others revealed that p116 Rip facilitates myosin light chain phosphatase (MLCP) activity through direct and indirect manners. However, it is unclear how p116 Rip regulates myosin phosphatase activity in cells. To elucidate the role of p116 Rip in cellular contractile processes, we suppressed the expression of p116 Rip by RNA interference in human airway smooth muscle cells (HASMCs). We found that knockdown of p116 Rip in HASMCs led to increased di‐phosphorylated MLC (pMLC), that is phosphorylation at both Ser19 and Thr18. This was because of a change in the interaction between MLCP and myosin, but not an alteration of RhoA/ROCK signaling . Attenuation of Zipper‐interacting protein kinase (ZIPK) abolished the increase in di‐pMLC, suggesting that ZIPK is involved in this process. Moreover, suppression of p116 Rip expression in HASMCs substantially increased the histamine‐induced collagen gel contraction. We also found that expression of the p116 Rip was decreased in the airway smooth muscle tissue from asthmatic patients compared with that from non‐asthmatic patients, suggesting a potential role of p116 Rip expression in asthma pathogenesis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".