Bioinformatic and morphological characterization of Catharanthus roseus mutants
Bibliographic record
Abstract
Catharanthus roseus, a member of the Apocynaceae family, has been studied extensively for its valuable chemotherapeutic monoterpenoid indole alkaloids (MIAs). Ethyl methanesuphonate (EMS) mutagenesis is a screening tool that has been used to look for altered MIA profiles in hope of discovering mutations of crucial MIA biosynthetic genes. Without a high-throughput mutation detection screen for C. roseus sequencing data, a range of techniques must be used to discover the EMS-induced changes within the plant. Bioinformatic and morphological analysis revealed the likely alterations leading to unique MIA profiles in two C. roseus EMS mutants: the high-ajmalicine accumulating line M2-0754 and the low-MIA accumulating line M2-1582. Expression of geissoschizine synthase (GS) was downregulated almost seven-fold in the leaves of M2-0754, leading to the accumulation of an alternate pathway MIA from the labile intermediate. The low-MIA profile and increased auxin sensitivity of M2-1582 is likely due to the expression of a dysfunctional auxin influx transport protein homologue.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".