Cortactin is a component of tubulobulbar complexes in Sertoli cells
Bibliographic record
Abstract
Tubulobulbar complexes (TCs) are double membrane tubular structures that project into Sertoli cells from sites of intercellular attachment. Large double membrane vesicles bud from the ends of the complexes and are degraded by Sertoli cells. TCs have been proposed to be part of the mechanism by which intercellular junctions are disassembled during sperm release and during the movement of spermatocytes from basal to adluminal compartments of the seminiferous epithelium. TCs resemble podosomes that develop at sites of cell attachment to substrate in other systems. The morphogenesis of podosomes is thought to be regulated by the interaction of the Arp2/3 complex, dynamin and cortactin. Although we previously have determined that the Arp2/3 complex and dynamin 3 are present at TCs, it is not known if cortactin also occurs at the sites. To determine whether or not cortactin is present at TCs we immunolabelled fixed frozen sections and fixed fragments of seminiferous epithelium with an antibody to cortactin. Intense labeling of TCs was present in sections and in epithelial fragments. Weak staining of ectoplasmic specializations also was detected. Controls were negative and immunoblots of testis and seminferous epithelium indicated that the antibody was specific. We conclude that cortactin is a component of TCs and therefore is a candidate regulator of TC morphogenesis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".