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Record W2974323710 · doi:10.1101/778183

Cell type-specific enhancer-promoter connectivity maps in the human brain and disease risk association

2019· preprint· en· W2974323710 on OpenAlexaff
Alexi Nott, Inge R. Holtman, Nicole G. Coufal, Johannes C. M. Schlachetzki, Miao Yu, Rong Hu, Claudia Z. Han, Monique Pena, Jiayang Xiao, Yin Wu, Zahara Keuelen, Martina P. Pasillas, Carolyn O’Connor, Simon T. Schafer, Zeyang Shen, Robert A. Rissman, James B. Brewer, David Gosselin, David Gonda, Michael L. Levy, Michael G. Rosenfeld, Graham McVicker, Fred H. Gage, Bing Ren, Christopher K. Glass

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2019
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSingle-cell and spatial transcriptomics
Canadian institutionsCentre hospitalier de l'Université LavalCentre hospitalier universitaire de Québec
FundersNational Center for Advancing Translational SciencesUniversity of California, San DiegoNational Cancer InstituteNational Institutes of HealthNederlandse Organisatie voor Wetenschappelijk OnderzoekJPB FoundationCure Alzheimer's FundCancer Research InstituteAlzheimer's AssociationAmerican Heart AssociationHartwell Foundation
KeywordsEnhancerCell typeBiologyGenome-wide association studyMicrogliaGeneHuman brainInteractomePromoterRegulatory sequenceGeneticsRegulation of gene expressionNeuroscienceComputational biologyCellTranscription factorGene expressionSingle-nucleotide polymorphismImmunologyGenotype

Abstract

fetched live from OpenAlex

Abstract Unique cell type-specific patterns of activated enhancers can be leveraged to interpret non-coding genetic variation associated with complex traits and diseases such as neurological and psychiatric disorders. Here, we have defined active promoters and enhancers for major cell types of the human brain. Whereas psychiatric disorders were primarily associated with regulatory regions in neurons, idiopathic Alzheimer’s disease (AD) variants were largely confined to microglia enhancers. Interactome maps connecting GWAS variants in cell type-specific enhancers to gene promoters revealed an extended microglia gene network in AD. Deletion of a microglia-specific enhancer harboring AD-risk variants ablated BIN1 expression in microglia but not in neurons or astrocytes. These findings revise and expand the genes likely to be influenced by non-coding variants in AD and suggest the probable brain cell types in which they function. One Sentence Summary Identification of cell type-specific regulatory elements in the human brain enables interpretation of non-coding GWAS risk variants.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.502
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.211
Teacher spread0.200 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations8
Published2019
Admission routes1
Has abstractyes

Explore more

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