Cloing and analyzing of cDNA for ethylene receptor, lcersi, of lychee (Litchi chinensis)
Bibliographic record
Abstract
The cDNA for an ethylene receptor, LcERS1, of Lychee (Litchi chinensis Sonn.) was obtained by a combination of RT-PCR and RACE technique. The length of cloned cDNA was 2, 193 bp, which include an Open Reading Frame of 1, 848 bp,from No.87 to No. 1934, encoding 615 amino acids, and about 68kD molecular weights. Genbank accession number for LcERS1 is AY311484. The putative protein has three conserved regions, one of them has the homologue with the histidine kinase A (phosphoreceptor) region, dimerisation and phsphoacceptor domain of histidine kinases. These regions are typical phosphotransfer site in the signal transduction system. The other region is GAF domain, which has more positives with the ethylene receptor gene (ers). The analysis of the protein structure and function suggested this putative protein be similar to the ethylene receptor gene, histidine protein kinase and the kinase region of the bacterium two-component signaling. The H and F motifs of the ethylene receptor genes were observed in the kinase region of the putative protein of lychee, they lies in 423-432 amino acid residues and 532-543 the amtno acid residues respectively. The conserved, sequence of His MNHE.MRTPM. and F is LMQTILNTMGA.. The putatve protein we obtained from lychee has the typical characters of ethylene receptor gene, which including three transmembrane regions in the N-terminal, one conserved histidine kinase domain and one GAF domain in the C-terminal. We strongly suggested that the cDNA sequence from lychee be the full length cDNA gene of the ethylene receptor gene (ers).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".