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Record W2979288789

Preliminary analysis of WND1B promoter from Populus trichocarpa

2012· article· en· W2979288789 on OpenAlexaff
Gangbiao Xu

Bibliographic record

VenueZhongnan Linye Keji Daxue xuebao · 2012
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant Gene Expression Analysis
Canadian institutionsL'Alliance Boviteq
Fundersnot available
KeywordsPopulus trichocarpaGeneTATA boxPromoterBiologyGeneticsUntranslated regionRegulatory sequenceStart codonArabidopsisMolecular biologyGene expressionMessenger RNAGenomeMutant
DOInot available

Abstract

fetched live from OpenAlex

WND1B gene from Populus trichocarpa is homologous to SND1,which is a key regulator of secondary wall synthesis in fiber cells of Arabidopsis.The 5' regulatory region of WND1B initiation codon advanced position about 2100 bp was cloned by PCR.TATA box,CAAT box and other core configurations were found in this promoter region.Several sequences similar to eukaryotic cis-regulatory elements were found in the 5'UTR proximal 5' flanking sequence.A series of truncated sequences of WND1B promoter were generated.The expression vectors pCAMBIA1301POG-1BP/1BP-F2/1BP-F3/1BP-F4-GUS were constructed and the transient expression analysis was carried out.The GUS activity determination revealed that the fragment of PtrWND1BP/ 1BP-F3/1BP-F4 could drive expression of the GUS gene while PtrWND1BP-F2 could not.The results suggested that the 5'UTR of PtrWND1B affected for the promoter activity.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.070
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.253
Teacher spread0.242 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2012
Admission routes1
Has abstractyes

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