Genetic Diversity of African Mistletoe (<i>Loranthus micranthus</i> Linn.) Accessions from Selected Plant Hosts Using Internal Transcribed Spacer (<i>ITS</i>) Gene
Bibliographic record
Abstract
African mistletoe ( Loranthus micranthus Linn.) is a parasitic plant which has shown to contain biologically active agents that have disease curing effects on humans. The present study aimed at evaluating the genetic diversity of L. micranthus accessions established on selected plant host based on ITS gene. 9 samples were selected for PCR amplification and sequencing of the its 1 and its 4 regions. The results revealed 104 and 89 polymorphic sites for its1 and its4 respectively. Haplotype diversity of 0.971+0.064 and nucleotide diversity 0.174+0.071 were recorded in its1 while haplotype diversity of 0.917+0.092 and nucleotide diversity of 0.231+0.027 were recorded in its4 . Phylogenetic analysis grouped the samples into 2 clusters based on the its regions. A high genetic distance (0.597) for its1 was obtained between samples from Pepper fruit and samples obtained from other host trees (African apple, Avocado and Monkey kola) while a low genetic distance (0.000) was obtained between C. apiculatum (LMV9) and African apple host (LMV4). The highest genetic distance (0.827) for its4 was obtained between C. apiculatum (LMV9) and African apple host (LMV4) while the lowest genetic distance (0.000) was obtained between Rubber hosts (LMV1, LMV2) and Pepper fruit host (LMV8). The results revealed some genetic diversity amongst the mistletoe grown on different hosts suggesting that host trees and geographical location might contribute to genetic diversity. It also showed that its1 and 4 regions differ in their variation discernment pattern in mistletoe and similar studies can be conducted in larger populations to accurately link genetic similarity/variation to host specificity and geographical origins.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".